Potential Effects of Cadmium Chloride and Glyphosate on DNA Damage in Nile tilapia
Ibrahim, I., Said, N., Abd-allah, M., Ramadan, A., Abdel-karim, E. (2018). Potential Effects of Cadmium Chloride and Glyphosate on DNA Damage in Nile tilapia. EKB Journal Management System, 14(1), 313-328. doi: 10.21608/blj.2018.285312
Ibrahim H. Ibrahim; Noha M. Said; Mohammed M. Abd-allah; Ashraf A. Ramadan; Esam A. Abdel-karim. "Potential Effects of Cadmium Chloride and Glyphosate on DNA Damage in Nile tilapia". EKB Journal Management System, 14, 1, 2018, 313-328. doi: 10.21608/blj.2018.285312
Ibrahim, I., Said, N., Abd-allah, M., Ramadan, A., Abdel-karim, E. (2018). 'Potential Effects of Cadmium Chloride and Glyphosate on DNA Damage in Nile tilapia', EKB Journal Management System, 14(1), pp. 313-328. doi: 10.21608/blj.2018.285312
Ibrahim, I., Said, N., Abd-allah, M., Ramadan, A., Abdel-karim, E. Potential Effects of Cadmium Chloride and Glyphosate on DNA Damage in Nile tilapia. EKB Journal Management System, 2018; 14(1): 313-328. doi: 10.21608/blj.2018.285312

Potential Effects of Cadmium Chloride and Glyphosate on DNA Damage in Nile tilapia

Biochemistry Letters
Volume 14, Issue 1, 2018, Page 313-328  XML PDF (802.53 K)
DOI: 10.21608/blj.2018.285312
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Authors
Ibrahim H. Ibrahim1; Noha M. Said2; Mohammed M. Abd-allah3; Ashraf A. Ramadan1; Esam A. Abdel-karim1
1Fish Genetics & Breeding Department, Central Laboratory for Aquaculture Research, Abbassa, Agriculture Research Center, Egypt.
2Biochemistry division, Chemistry Department, Faculty of Sciences, Zagazig University, Zagazig, Sharkia, Egypt.
3Chemistry Department, Faculty of Sciences, Zagazig University, Zagazig, Sharkia, Egypt.
Abstract
The present study was conducted to estimate the effect of cadmium chloride as a heavy metal and commercial glyphosate (Roundup®) as a herbicide on DNA damage as biochemical and molecular biomarkers in the Nile tilapia, Oreochromis niloticus through three exposure periods with different concentrations of LC50. The 96h-LC50 were determined for CdCl2 (132mg/l), glyphosate (9.63mg/l), CdCl2 in mixture (41.30mg/l) and glyphosate in mixture (2.75mg/l), respectively. The fish exposed to these concentrations separately and mixed for 4 days as well as two sublethal concentrations (1/4 and 1/10 LC50) for 8 days and 45 days, respectively. Gills and liver cells of the exposed samples were taken after 4, 8 and 45 days to examine the DNA damage using comet assay. The highest DNA damage was observed on gills after 4 days in treatment of mixture due to presence of synergistic effect between CdCl2 and glyphosate , after 8 days in treatment of glyphosate and after 45 days in treatment of cadmium. The highest DNA damage was observed on liver after 4days and 8days in treatment of glyphosate but after 45days in treatments of cadmium and mixture. DNA damage depends on concentration of pollutants and exposure periods.
Keywords
Nile tilapia; Glyphosate; Cadmium chloride; Molecular biomarker; Comet assay; DNA damage
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