Risk Prediction Genetic Study of Non-HLA Gene NOD2 Three Polymorphisms in Acute Graft versus Host Disease after Allogeneic Hematopoietic Stem Cell Transplantation
Ibrahim, H., Ghanem, A., Shehata, M., Fathy, G. (2018). Risk Prediction Genetic Study of Non-HLA Gene NOD2 Three Polymorphisms in Acute Graft versus Host Disease after Allogeneic Hematopoietic Stem Cell Transplantation. EKB Journal Management System, 27(3), 135-142. doi: 10.21608/ejmm.2018.285602
Hanan F. Abd El-Aziz Ibrahim; Azaa M. Shawky Ghanem; Maha I. Shehata; Gamal EL Din M. Fathy. "Risk Prediction Genetic Study of Non-HLA Gene NOD2 Three Polymorphisms in Acute Graft versus Host Disease after Allogeneic Hematopoietic Stem Cell Transplantation". EKB Journal Management System, 27, 3, 2018, 135-142. doi: 10.21608/ejmm.2018.285602
Ibrahim, H., Ghanem, A., Shehata, M., Fathy, G. (2018). 'Risk Prediction Genetic Study of Non-HLA Gene NOD2 Three Polymorphisms in Acute Graft versus Host Disease after Allogeneic Hematopoietic Stem Cell Transplantation', EKB Journal Management System, 27(3), pp. 135-142. doi: 10.21608/ejmm.2018.285602
Ibrahim, H., Ghanem, A., Shehata, M., Fathy, G. Risk Prediction Genetic Study of Non-HLA Gene NOD2 Three Polymorphisms in Acute Graft versus Host Disease after Allogeneic Hematopoietic Stem Cell Transplantation. EKB Journal Management System, 2018; 27(3): 135-142. doi: 10.21608/ejmm.2018.285602

Risk Prediction Genetic Study of Non-HLA Gene NOD2 Three Polymorphisms in Acute Graft versus Host Disease after Allogeneic Hematopoietic Stem Cell Transplantation

Egyptian Journal of Medical Microbiology
Volume 27, Issue 3, July 2018, Page 135-142  XML PDF (386.2 K)
Document Type: New and original researches in the field of Microbiology.
DOI: 10.21608/ejmm.2018.285602
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Authors
Hanan F. Abd El-Aziz Ibrahim email 1; Azaa M. Shawky Ghanem1; Maha I. Shehata1; Gamal EL Din M. Fathy2
1Microbiology and Immunology Department, Faculty of Medicine for Girls, Al-Azhar University, Cairo-Egypt
2Hematology and Bone Marrow Transplantation Department, Naser Institute, Cairo-Egypt
Abstract
Background: Graft-versus-host disease (GVHD) has been associated with non- human leukocyte antigen (HLA) gene polymorphisms after allogenic Hematopoietic stem cell transplantation (aHSCT). Objective: This study aims to investigate the role of three polymorphisms in Nucleotide-binding Oligomerisation Domain (NOD2) gene on the occurrence / severity of acute GVHD among Egyptian population. Methodology: A total of 64 patients and their corresponding donors who underwent aHSCT from HLA-identical matched siblings were genotyped by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). Results were confirmed by automated laser sequencer. Results: There was highly statistical significant association between NOD2 recipient l007fs allele and higher incidence of aGVHD p= 0.006. There was no significant association between NOD2 donor l007fs allele, G908R allele in donors and recipients, R702W in donors and recipients with incidence and severity of aGVHD. Conclusion: This research an original pilot study to demonstrate that the NOD2 SNP13 (Leu1007fs) is a possible risk factor for aGVHD among Egyptian population.
Keywords
NOD2 polymorphism; GVHD; aHSCT; PCR-RFLP
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