FimH Adhesin among Enterobacter spp. Isolates and its Relation to Biofilm Formation and Antimicrobial Resistance Pattern
Ghonaim, M., Elkhyat, A., El-Hefnawy, S., Hossam Eldeen, E. (2018). FimH Adhesin among Enterobacter spp. Isolates and its Relation to Biofilm Formation and Antimicrobial Resistance Pattern. EKB Journal Management System, 27(4), 45-54. doi: 10.21608/ejmm.2018.285640
Mabrouk M Ghonaim; Amira H Elkhyat; Sally M El-Hefnawy; Elham A Hossam Eldeen. "FimH Adhesin among Enterobacter spp. Isolates and its Relation to Biofilm Formation and Antimicrobial Resistance Pattern". EKB Journal Management System, 27, 4, 2018, 45-54. doi: 10.21608/ejmm.2018.285640
Ghonaim, M., Elkhyat, A., El-Hefnawy, S., Hossam Eldeen, E. (2018). 'FimH Adhesin among Enterobacter spp. Isolates and its Relation to Biofilm Formation and Antimicrobial Resistance Pattern', EKB Journal Management System, 27(4), pp. 45-54. doi: 10.21608/ejmm.2018.285640
Ghonaim, M., Elkhyat, A., El-Hefnawy, S., Hossam Eldeen, E. FimH Adhesin among Enterobacter spp. Isolates and its Relation to Biofilm Formation and Antimicrobial Resistance Pattern. EKB Journal Management System, 2018; 27(4): 45-54. doi: 10.21608/ejmm.2018.285640

FimH Adhesin among Enterobacter spp. Isolates and its Relation to Biofilm Formation and Antimicrobial Resistance Pattern

Egyptian Journal of Medical Microbiology
Volume 27, Issue 4, October 2018, Page 45-54  XML PDF (576.38 K)
Document Type: New and original researches in the field of Microbiology.
DOI: 10.21608/ejmm.2018.285640
View on SCiNiTO View on SCiNiTO
Authors
Mabrouk M Ghonaim1; Amira H Elkhyat1; Sally M El-Hefnawy2; Elham A Hossam Eldeen email 3
1Departments of Microbiology and Immunology, Faculty of Medicine, Menoufia University, Shebin Elkom, Egypt
2Departments of Biochemistry, Faculty of Medicine, Menoufia University, Shebin Elkom, Egypt
3Departments of Microbiology and Immunology, Faculty of Medicine, Menoufia University, Shebin Elkom, Egypt
Abstract
Background: Enterobacter spp., which are able to carry a number of antibiotic-resistance genes, can cause a variety of infections especially in immune-compromised individuals and patients from intensive care units (ICUs). Objectives: To assess the prevalence of Enterobacter spp. in Menoufia University Hospitals, and to investigate the relation between antimicrobial susceptibility patterns and biofilm production. Methodology: A total 296 clinical samples from patients admitted to Menoufia University Hospitals. Enterobacter spp. were identified by standard microbiological methods and Vitek-2 system. All Enterobacter isolates antibiogram was tested by the modified Kirby Bauer disk diffusion method, and for extended-spectrum β-lactamases and metallo-β-lactamase production. Biofilm production was detected by congo red agar, modified congo red agar methods and PCR. Results: Enterobacter spp. represented 17.3% of all the collected nosocomial isolates. Vitek-2 system showed that the predominant spp. was Enterobacter aerogenes (44%). Enterobacter isolates were resistant to amoxicillin (100%), doxycycline (82%) and gentamycin (76%). The rates of resistance to ceftriaxone, cefoxitin, cefepime, and amikacin were 64%, 72%, 60% and 70% respectively. Half of Enterobacter isolates were sensitive to piperacillin/tazobactam, meropenem, ciprofloxacin, ofloxacin and norfloxacin while 84% were sensitive to chloramphenicol. Production of ESβLs and MβL was found among 28% and 22% of isolates respectively. Biofilm production was found among 50% by CRA method and 56% by MCRA method, while conventional PCR showed fimH gene among 58% of Enterobacter isolates. Conclusion: Enterobacter spp. are serious nosocomial pathogens as they can produce ESβLs and carbapenemase, and produce biofilm that is related to their antimicrobial resistance. Therefore, their adequate prevention and control is imperative.
Keywords
Nosocomial infections; Enterobacter; biofilm; fimH gene
Statistics
Article View: 84
PDF Download: 52