Identification of faba bean (Vicia faba L.) protein by electrophoresis

Yassen, A. A. M.; Taha, M. G. A.; Ibrahim, M. M.; Khalifa, Y. A. M.

doi:10.21608/aasj.2022.286228

	Identification of faba bean (Vicia faba L.) protein by electrophoresis
Archives of Agriculture Sciences Journal
Article 38, Volume 5, Issue 3, December 2022, Page 261-274 PDF (432.4 K)
Document Type: Research article
DOI: 10.21608/aasj.2022.286228
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Authors
A. A. M. Yassen ¹; M. G. A. Taha²; M. M. Ibrahim¹; Y. A. M. Khalifa¹
¹Department of Agronomy-Biochemistry, Faculty of Agriculture, Al-Azhar University, Assiut Branch, Egypt
²Department of Agriculture-Biochemistry, Faculty of Agriculture, Al-Azhar University, Cairo, Egypt
Abstract
The seeds of fifteen faba bean varieties and genotypes were used in this study; Giza 716, Giza 843, Noubariaa 3, Wady 1, Sakha 1, T. W, hybrid 21, hybrid 22, hybrid 23, hybrid 24, Sakha 4, Noubariaa 1, Giza 40, Missr 1 and Giza 3. The chemical composition of all the previously mentioned varieties and genotypes was done. Results indicated that, carbohydrate content ranged from 51.11-58.15%, protein content ranged from 24.60%-30.68%, moisture from 9.07-10.57%, fat content from 1.54- 2.70% and ash content from 1.38 - 4.16%. Proteins were fractionated by Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS–PAG). Results revealed that Giza 3, Giza 40, Sakha 4, Noubariaa 1 and T.W were resolved into 8 bands, Giza 716, Giza 843, hybrid 22 and Missr1 were resolved into 9 bands, while hybrid 24, hybrid 23, Wady 1, and Sakha 1 were resolved into 10 bands. Also, hybrid 21 and Noubariaa 3 were resolved into 11 bands. The molecular weight of protein subunits ranged between 15.00 and 352.50 kDa.
Keywords
faba bean; gel electrophoresis; SDS–PAG; molecular weight; dodecyl sulfate polyacrylamide


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