Bioremediation of Polluted Soil with Hydrocarbons by Some of Trichoderma Spp in Al-Najaf-Iraq | ||||
| The Egyptian Journal of Hospital Medicine | ||||
| Article 96, Volume 90, Issue 2, January 2023, Page 2620-2627 PDF (1.01 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejhm.2023.286409 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Nihad Habeeb Mutlag  ; Sara A. Hamoudi; Wafaa N. Radhi
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| Abstract | ||||
| Background: There are many types of microorganisms such as bacteria and fungi, which have the potential for biochemical analysis of hydrocarbons each organism has a specific role in the analysis process. Material and methods: This study was conducted by addition some of biocontrol fungi Trichoderma harzianum (isolate1), Trichoderma harzianum (isolate2), Trichoderma longibrachiatum (isolate 3) in the treatment of contaminated soil with oil derivatives including the addition of 40 ml of white oil (WO) into the experimental pot containers containing 400 gm of soils and mixed well to achieve artificial pollution. Then, three studied isolates of Trichoderma spp < /em>. were added and mixed with all soil samples that artificially prepared in addition to controlling pots (without the addition of WO) three replicates for each treatment. The biodegradation of Total Petroleum Hydrocarbons (TPH) %and Biostimulation Efficiency (B.E) % were calculated for 30,60,90 days after the treatment with bioagent fungi. Spectrophotometer technique was used to estimate the total petroleum hydrocarbons) that showed T. longibrachiatum isolate gave the highest Total Petroleum Hydrocarbons (TPH) % reduction in site 1 reaching (93.2%) after 90 days. Result: Trichoderma (isolate 2) has high rate of biostimulation efficiency% that reach (94.2%). Scanning Electronic Microscope (SEM) was used to compare the vegetative growth of fungi before and after the addition hydrocarbon with PDA media, which showed the clear and normal growth with short period to the growth in petri dish after addition 1ml of oil in comparison with the control petri dish in the three magnification forces values (1000x, 2000x, 5000x, 6000x, 10000x, 14000x) for all three studied bioagent fungi before and after the addition of oil. Conclusion: Trichoderma has high rate of biostimulation efficiency, before and after the addition hydrocarbon with PDA media by using of SEM showed the clear and normal growth with short period to the growth in petri dish after addition 1ml of oil in comparison with the control petri dish in the three magnification forces values. | ||||
| Keywords | ||||
| Bioremediation; Trichoderma; Total Hydrocarbons; SEM; Biocontrol Fungi | ||||
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