The Possible Toxic Effects of Glutathione Intramuscular Injection on Ovaries of Female Albino Rats (Experimental Study)

Kamel, Mariam Kamal; Habib, Sahar Refaat; Abdel Zaher, Azza Mohamed; Mohamed Fathi, Nada Ahmed Yousri

doi:10.21608/mjmr.2023.181492.1320

	The Possible Toxic Effects of Glutathione Intramuscular Injection on Ovaries of Female Albino Rats (Experimental Study)
Minia Journal of Medical Research
Articles in Press, Accepted Manuscript, Available Online from 20 February 2023
Document Type: Original Article
DOI: 10.21608/mjmr.2023.181492.1320
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Authors
Mariam Kamal Kamel ¹; Sahar Refaat Habib¹; Azza Mohamed Abdel Zaher²; Nada Ahmed Yousri Mohamed Fathi¹
¹Forensic medicine and clinical toxicology department, Faculty of Medicine, Minya University, Egypt
²Pathology department, Faculty of Medicine, Minya University, Egypt
Abstract
Background and objective: Glutathione can alter the depigmentation of skin and convert eumelanin to pheomelanin during melanogenesis. As a result, glutathione has become one of the most used skin-whitening agents, and women have become obsessed with it. So, the purpose of this study was to see how intramuscular glutathione injection can affect the ovaries of female albino rats. Methods: 50 female albino rats were involved. The animals were divided into two groups; A: the control group and B: the glutathione-injected group, which received glutathione intramuscularly three times per week for three consecutive weeks at a dose of 30 mg/day. The injected group was subdivided into four subgroups based on how soon after the last dose the rats were sacrificed (B1: immediately, B2: after 24h, B3: after one week and B4: after two weeks). Ovarian tissue samples were collected from all groups. Each sample was subjected for histopathological examination and estimation of estrogen and progesterone by ELISA. Malondialdehyde (MDA) and reduced glutathione (GSH) levels also were detected. Results: MDA level was significantly higher in subgroups of group B than group A, while GSH level was significantly reduced. The histopathological examination revealed that glutathione injections led to edematous stroma and luteoma. As regard hormones levels, there was significant increase in all (B) subgroups compared to control group. In this study, ovarian changes caused by the prooxidant behavior of glutathione, were reduced partially after two weeks. Conclusion: External glutathione may function as a pro-oxidant and have negative effect on the ovaries.
Keywords
Estrogen; Progesterone; ELISA and MDA


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