Association study of uncoupling protein 2-866G/A (rs659366) gene polymorphism and serum myeloperoxidase with cardiovascular disease in type 2 diabetic patients
Farhan, H., Abo Gabl, K., Mandour, I., Baz, H., Khalil, N., Nassar, M., Gabr, M. (2023). Association study of uncoupling protein 2-866G/A (rs659366) gene polymorphism and serum myeloperoxidase with cardiovascular disease in type 2 diabetic patients. EKB Journal Management System, 4(1), 114-129. doi: 10.21608/ejmr.2022.181672.1303
Hanan Mohamed Farhan; Khadiga Abo Gabl; Iman Atef Mandour; Heba Nabil Baz; Neveen Ahmed Khalil; Mahmoud Nassar; Mohamed aly Gabr. "Association study of uncoupling protein 2-866G/A (rs659366) gene polymorphism and serum myeloperoxidase with cardiovascular disease in type 2 diabetic patients". EKB Journal Management System, 4, 1, 2023, 114-129. doi: 10.21608/ejmr.2022.181672.1303
Farhan, H., Abo Gabl, K., Mandour, I., Baz, H., Khalil, N., Nassar, M., Gabr, M. (2023). 'Association study of uncoupling protein 2-866G/A (rs659366) gene polymorphism and serum myeloperoxidase with cardiovascular disease in type 2 diabetic patients', EKB Journal Management System, 4(1), pp. 114-129. doi: 10.21608/ejmr.2022.181672.1303
Farhan, H., Abo Gabl, K., Mandour, I., Baz, H., Khalil, N., Nassar, M., Gabr, M. Association study of uncoupling protein 2-866G/A (rs659366) gene polymorphism and serum myeloperoxidase with cardiovascular disease in type 2 diabetic patients. EKB Journal Management System, 2023; 4(1): 114-129. doi: 10.21608/ejmr.2022.181672.1303

Association study of uncoupling protein 2-866G/A (rs659366) gene polymorphism and serum myeloperoxidase with cardiovascular disease in type 2 diabetic patients

Egyptian Journal of Medical Research
Volume 4, Issue 1, January 2023, Page 114-129  XML PDF (528.42 K)
Document Type: Original Article
DOI: 10.21608/ejmr.2022.181672.1303
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Authors
Hanan Mohamed Farhan email 1; Khadiga Abo Gabl1; Iman Atef Mandour2; Heba Nabil Baz2; Neveen Ahmed Khalil1; Mahmoud Nassarorcid 3; Mohamed aly Gabr4
1Clinical & Chemical Pathology Department, Faculty of Medicine, Beni-Suef University, Beni-Suef, Egypt
2Clinical & Chemical Pathology Department, Faculty of Medicine, Cairo University, Cairo, Egypt
3Internal Medicine Department, Icahn School of Medicine at Mount Sinai, NYC Health+Hospital, Queens, New York, USA
4Chest Unit, Ahmed Maher Teaching Hospital, Cairo, Egypt
Abstract
Diabetic cardiovascular diseases are leading causes of death. In diabetic patients, oxidative stress is key event in cardiovascular disease (CVD) development. Uncoupling protein 2 (UCP2) and myeloperoxidase (MPO) were identified as molecules regulating oxidative stress effects of oxygen free radicals. This work aimed to evaluate association of UCP2-866G/A (rs659366) gene polymorphism and serum MPO with CVD among type 2 diabetic patients. The present work was conducted on 80 type 2 diabetic Egyptian patients including 40 patients with CVD compared to 40 age- and sex-matched non-CVD patients; recruited from Outpatient Clinics in Beni-suef University Hospital and Kasr Eleiny Hospital. Lipid profile was assayed. Serum MPO was analyzed using enzyme-linked immunosorbent assay. UCP2-866G/A (rs659366) gene polymorphism was determined using polymerase chain reaction-restriction fragment length polymorphism. Statistically significant increase in concentration of total cholesterol (TC) and low density lipoprotein cholesterol (LDL-C) was found among CVD patients with MPO ˃ 44.3 ng/ml (P=0.01 and 0.019, respectively). Serum MPO showed significant positive correlation with TC and LDL-C (P=0.004 and 0.002, respectively). The UCP2-866 (rs659366) (GA and AA) genotype variants did not demonstrate statistically significant higher risk to develop cardiovascular events, yet were observed to have higher frequency among CVD patients and among patients with MPO ˃ 44.3ng/ml. In conclusion, our results revealed significant correlation between serum myeloperoxidase and CVD lipid risk factors in diabetic patients. This study suggests UCP2 -866G/A (rs659366) gene polymorphism and MPO as target assay studies on CVD etiology and oxidative stress parameters evaluation among type 2 diabetic patients.
Keywords
Cardiovascular disease; oxidative stress; myeloperoxidase; uncoupling protein 2; UCP2-866G/A (rs659366) gene polymorphism
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