Effect Of High Fat Diet Induced Metabolic Syndrome And Treatment With Insulin Like Growth Factor 1 On Biochemical And Immunohistochemical Parameters Related To Neuronal Functions In Adult Male Albino Rats.
Yousuf, A., Elshora, S., Abdallah, M., Elmongy, N., Aboelsoud, H., Mosallam, H., Algendy, A., Abd El-Lateef, A., El-Sharkawy, A., Seliem, N., El shahat, R., Alkot, A. (2023). Effect Of High Fat Diet Induced Metabolic Syndrome And Treatment With Insulin Like Growth Factor 1 On Biochemical And Immunohistochemical Parameters Related To Neuronal Functions In Adult Male Albino Rats.. EKB Journal Management System, 66(13), 389-400. doi: 10.21608/ejchem.2023.191284.7550
Asmaa Fathy Yousuf; Shimaa Zakaria Elshora; Mohamed Gaber Abdallah; Noura F. Elmongy; Heba Abdelnaser Aboelsoud; Hala Hamed Mosallam; Ashraf Mohamed Mohamed Algendy; Abd El-Lateef Saeed Abd El-Lateef; Abdou Mohammed El-Sharkawy; Nora Seliem; Rehab Mohamed El shahat; Ahmad Mohammad Farag Alkot. "Effect Of High Fat Diet Induced Metabolic Syndrome And Treatment With Insulin Like Growth Factor 1 On Biochemical And Immunohistochemical Parameters Related To Neuronal Functions In Adult Male Albino Rats.". EKB Journal Management System, 66, 13, 2023, 389-400. doi: 10.21608/ejchem.2023.191284.7550
Yousuf, A., Elshora, S., Abdallah, M., Elmongy, N., Aboelsoud, H., Mosallam, H., Algendy, A., Abd El-Lateef, A., El-Sharkawy, A., Seliem, N., El shahat, R., Alkot, A. (2023). 'Effect Of High Fat Diet Induced Metabolic Syndrome And Treatment With Insulin Like Growth Factor 1 On Biochemical And Immunohistochemical Parameters Related To Neuronal Functions In Adult Male Albino Rats.', EKB Journal Management System, 66(13), pp. 389-400. doi: 10.21608/ejchem.2023.191284.7550
Yousuf, A., Elshora, S., Abdallah, M., Elmongy, N., Aboelsoud, H., Mosallam, H., Algendy, A., Abd El-Lateef, A., El-Sharkawy, A., Seliem, N., El shahat, R., Alkot, A. Effect Of High Fat Diet Induced Metabolic Syndrome And Treatment With Insulin Like Growth Factor 1 On Biochemical And Immunohistochemical Parameters Related To Neuronal Functions In Adult Male Albino Rats.. EKB Journal Management System, 2023; 66(13): 389-400. doi: 10.21608/ejchem.2023.191284.7550

Effect Of High Fat Diet Induced Metabolic Syndrome And Treatment With Insulin Like Growth Factor 1 On Biochemical And Immunohistochemical Parameters Related To Neuronal Functions In Adult Male Albino Rats.

Egyptian Journal of Chemistry
Volume 66, Issue 13, December 2023, Page 389-400  XML PDF (1.86 MB)
Document Type: Original Article
DOI: 10.21608/ejchem.2023.191284.7550
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Authors
Asmaa Fathy Yousuforcid 1; Shimaa Zakaria Elshoraorcid 2; Mohamed Gaber Abdallahorcid 3; Noura F. Elmongyorcid 4; Heba Abdelnaser Aboelsoudorcid 5; Hala Hamed Mosallam5; Ashraf Mohamed Mohamed Algendyorcid 6; Abd El-Lateef Saeed Abd El-Lateeforcid 7; Abdou Mohammed El-Sharkawy7; Nora Seliemorcid 8; Rehab Mohamed El shahatorcid 9; Ahmad Mohammad Farag Alkot email orcid 10
1Medical physiology department, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt
2Histology department, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt
3Department of Medical Biochemistry, Faculty of Medicine, Al-Azhar University, Cairo, Egypt.
4Medical physiology department, Damietta Faculty of Medicine, Al-Azhar University, Damietta, Egypt
5Anatomy and Embryology department, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt
6Department of Medical Physiology, Faculty of Medicine, Al-Azhar University, Cairo, Egypt
7Department of Medical Pharmacology, Faculty of Medicine, Al-Azhar University, Cairo, Egypt.
8Biochemistry department, faculty of medicine for girls, Al-Azhar University, Cairo, Egypt
9Pharmacology department, Faculty of medicine for girls, Al- Azhar University, Cairo, Egypt
10Medical Physiology Department, Faculty of Medicine, Al-Azhar University, Cairo, Egypt
Abstract
The prevalence of metabolic syndrome (MetS) makes type 2 diabetes mellitus and its consequences more common in communities. Dysfunctions of the nervous system has negative implications on the lives of affected individuals and their families. This study aimed to elucidate the possible role of MetS and treatment with in insulin like growth factor 1 (IGF-1) on some biochemical and immunohistochemical parameters related to neuronal functions in adult male albino rats. For this purpose, fifty adult male albino rats were recruited and divided into five groups. (1) control group, (2) MetS group (induced by high fat diet for eight weeks), (3) MetS group treated with IGF-1 for six weeks started two weeks before induction of MetS, (4) MetS group treated with IGF-1 for six weeks started with induction of MetS, (5) MetS group treated with IGF-1 for six weeks started two weeks after starting induction of MetS. Fasting blood glucose (FBG), insulin, lipid profile, glucagon like peptide 1 (GLP1), tumor necrosis factor-α (TNF-α) and adiponectin levels were measured in the serum whereas malonaldehyde, glutathione peroxidase (GPx), brain derived neurotrophic factor (BDNF), synapsin 1 and sortilin were measured in brain tissue homogenate. Histo pathological and immunohistochemical studies for B cell lymphoma 2 (BCL2) and glial fibrillary acidic protein (GFAP) were also done. The results showed that induction of MetS caused hyperglycemia, hyperinsulinemia, dyslipidemia, elevation of the oxidant marker MDA and the proinflamatory TNF-α whereas the anti-oxidant GPx and the anti-infalimatory adiponectin were elevated. BDNF and the anti-apoptotic BCL2 were reduced whereas synapsin 1, sortilin and the astrocytes proliferation marker GFAP were increased. Treatment with IGF-1 was associated with improvement in all these parameters. It could be concluded that MetS is associated with reduction in neuronal protective factors and enhancement of factors which impair neuronal functions and structures. These effects may underlie the pathogenesis of cognitive dysfunction associating MetS. By antagonizing such effects of MetS, IGF-1 may be helpful in managing MetS and associated cognitive dysfunction.
Keywords
Metabolic syndrome; Cognitive dysfunction; Insulin like growth factor 1; Brain derived neurotrophic factor; synapsin 1; Sortilin
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