DNA profiling of Egyptian Astragalus spinosus (Forssk.) Muschl. species using AFLP markers | ||||
| Egyptian Journal of Pure and Applied Science | ||||
| Article 2, Volume 61, Issue 1, January 2023, Page 11-18 PDF (1.1 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejaps.2023.182549.1048 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Eman Ali   1; Naglaa Sherif1; EL-Shaimaa El-Demerdash 2; Rasha Hassan 1
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| 1Biochemistry Department, Faculty of Science, Ain Shams University, Cairo, Egypt | ||||
| 2Genetic Resources Department, Desert Research Centre, Cairo, Egypt | ||||
| Abstract | ||||
| Abstract: Background: Species of Astragalus have been used medicinally for more than a thousand years. Astragalus spinosus (Forssk.) Muschl. extract has been used in the treatment of various diseases. Despite its numerous and varied uses, studies on the genetic makeup of Egyptian A. spinosus are lacking. Therefore, the current study was conducted to generate a molecular fingerprint for this species in two successive growth periods which will be used to recognize, record, and act as a molecular reference for future research. Method: Astragalus spinosus samples were collected from El-Hawala region of Marsa-Matrouh, Egypt's North Coast and subjected to molecular analyses using the amplified fragment length polymorphism technique (AFLP) with four primer combinations. Results: Our results revealed minute variations among A. spinosus samples regardless of different collecting periods. Conclusions: The current work confirmed the accuracy and reliability of the AFLP technique in constructing an accurate genomic picture of the investigated plant. | ||||
| Keywords | ||||
| Keywords: AFLP; Astragalus spinosus; DNA fingerprinting | ||||
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