Detection of Neosporium Caninium in aborted fetuses in a dairy farm in Alexandria governorate | ||||
Egyptian Veterinary Medical Society of Parasitology Journal (EVMSPJ) | ||||
Volume 18, Issue 1, September 2022, Page 45-52 PDF (368.08 K) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/evmspj.2022.288597 | ||||
View on SCiNiTO | ||||
Author | ||||
Mohamed A. Soltan | ||||
Departement of Vet,Medicine (Infectious Diseases),Faculty of Vet.Medicine,Suez Canal University | ||||
Abstract | ||||
A dairy Holstein cattle farm complained of cases of abortion at late stages of pregnancy (7 to 9 months) 75/500 pregnant cows (15%). The farm regularly vaccinated for BVDV, IBR, and Leptospira with the cattle master vaccine (Zoetis). Additionally, no positive reactors were recorded during routine testing by the rose Bengal agglutination test for bovine brucellosis. A total of 3 aborted fetuses were sent to the infectious diseases laboratory, Suez Canal University for laboratory testing. The internal organs (Brain, liver, lung, and lymph nodes) were collected from the fetuses and tested by quantitative PCR for BVDV, BHV-1, Brucellosis, and leptospirosis; and nested PCR for Neopsorium caninum (N. caninum). The tested samples were negative for BVDV, BHV-1, Leptospira and Brucella but, positive for N. caninum. The further sequencing of positive samples confirmed infection by N. caninum. The farm owner stated that there are about 15 dogs reared on the same farm and their feces may contaminate the silage. Also, the cases of abortion decreased after removal of dogs from the farm | ||||
Keywords | ||||
Neospora; Fetus; Cows | ||||
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