Functional, Histological and Immunohistochemical Study on the Possible Effect of Vitamin D and mesenchymal stem cells on decreasing bone-remodeling changes in Streptozotocin Induced Diabetic Rats | ||||
| Egyptian Journal of Histology | ||||
| Articles in Press, Accepted Manuscript, Available Online from 08 March 2023 | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejh.2023.190512.1849 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Asharf Kotb1; Sara Hosny   2; Ahmed Desoky Badawy3; Fawzy Abd_ElTawab Abd_Ellatif1; Rania Elsayed Hussein4; Asmaa ShamsEldeen5
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| 1Department of Medical Physiology, Faculty of Medicine, October 6 University, Giza, Egypt. | ||||
| 2Histology department, faculty of medicine, cairo university | ||||
| 3physiology department October 6 University | ||||
| 4Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Egypt | ||||
| 5Department of Medical Physiology, Faculty of medicine, Cairo University Department of Medical Physiology, Faculty of Medicine, October 6 University, Giza, Egypt. | ||||
| Abstract | ||||
| Background and Objectives: Diabetes mellitus (DM) is a metabolic disorder associated with osteoporosis and increased risk of bone fracture. This study aimed to clarify the effect of diabetes mellitus on bone remodeling changes, and to study the role of vit D supplementation and mesenchymal stem cells (BM-MSCs) in decreasing bone remodeling changes in type II DM. Material and Methods: 30 adult male albino rats were classified into: Group I (n-6) (control group) and main experimental group (n=24). All rats in experimental group were fed high fat diet and injected once with streptozotocin (STZ) (45 mg/kg) intraperitoneally (ip) then after 8 weeks they were divided into; group II; continued without treatment, group III; received vitamin D orally daily, group IV; received once intravenous injection of 3 × 106 BM-MSCs and group V; treated with combined BM-MSCs and vitamin D. At the end of the experiment, Blood samples were collected to measure glucose level, lipid profile (FFA), reactive Oxygen species (ROS) and tumor Necrosis factor (TNF-α.). Femur bones were dissected for biochemical, histological, immunohistochemical, morphometric and statistical analysis. Results: Mean serum levels of glucose, triglycerides, total cholesterol (TCH), ROS & TNF-α were significantly increased associated with significant decrease of HDL & FFA level in groups II, III and IV. In addition, the levels of Wnt/ b-catenin and BCL2 were significantly decreased, while sclerostin and BAX were increased area percentage of VEGF in groups II, III and IV. Vitamin D and BM-MSCs encountered significant amelioration in biochemical markers, histological & immunohistochemical changes induced by STZ with a more obvious improvement in combined group (group V). Conclusion: Combined treatment of vitamin D and BM-MSCs significantly improved diabetic induced bone turnover compared with injection of MSCs or vit. D alone by alleviating the antioxidative and anti-inflammatory effects. | ||||
| Keywords | ||||
| Diabetes; bone turnover; Wnt/ b-catenin; BAX/BCL2; VEGF | ||||
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