Polymorphisms in the exon-3 of leptin gene using Tetra-primer ARMS-PCR in Egyptian buffalo | ||||
| Al-Azhar Journal of Agricultural Research | ||||
| Articles in Press, Accepted Manuscript, Available Online from 10 March 2023 | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ajar.2022.148215.1076 | ||||
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| Authors | ||||
Fahmy Ahmed Sharara  1; Mohamed Ahmed Aboul-Hassan1; Waleid Mohamed ٍShakweer2
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| 1Animal Production Department, Fac. of Agriculture, Al-Azhar University, Egypt | ||||
| 2Animal Production Department, Agricultural and Biological Research Institute, National Research Centre, 33 El-Buhouth Street, P.O: 12622, Dokki, Cairo, Egypt | ||||
| Abstract | ||||
| The Egyptian buffalo has a great importance in Egypt, because of its great adaptability to local harsh environmental conditions, resistance to infectious diseases, and the buffalo milk is preferred by Egyptian consumers because of its good flavor and composition. Leptin hormone has different functions, including modulation of reproduction, metabolism, and mammary gland development, as well as cell differentiation and proliferation. Leptin is primarily produced in adipose tissue and also expressed from other tissues, including the placenta, mammary glands, skeletal muscles, and pituitary glands. Tetra-primer ARMS-PCR (Tetra-primer ARMS), a simple and inexpensive SNP genotyping technique, allows for the simultaneous detection of alleles using a single PCR reaction and gel electrophoresis. Therefore, the aim of the present studies was to assess the allelic and genotypic frequency of SNP A G>A at codon 159 in Egyptian buffalo by Tetra-primer ARMS-PCR. A total of 74 blood samples were collected from Egyptian buffalo. Three genotypes for GG, AG, and AA with frequencies of 0.49, 0.39, and 0.12, respectively. Allele frequencies of G and A were found to be 0.69 and 0.31, respectively. For the first time, allelic and genotypic frequency of G>A SNP at codon 159 in Egyptian buffalo was detected, with the highest allelic frequency of allele A (0.31). Further studies are needed to provide more information about the association between this SNP and production and reproduction traits. | ||||
| Keywords | ||||
| Egyptian buffalo; Tetra-primer; Leptin | ||||
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