OREXIN-1-RECEPTOR BLOCKER, SB-334867 MAY AFFECT BODY WEIGHT AND PROTECT AGAINST HYPOGLYCEMIA INDUCED BY PARADOXICAL SLEEP DEPRIVATION IN ADULT MALE RATS
Ali, M., Moghazy, H., Mahmoud, A., AbdelSater, K. (2019). OREXIN-1-RECEPTOR BLOCKER, SB-334867 MAY AFFECT BODY WEIGHT AND PROTECT AGAINST HYPOGLYCEMIA INDUCED BY PARADOXICAL SLEEP DEPRIVATION IN ADULT MALE RATS. EKB Journal Management System, 39(1), 91-101. doi: 10.21608/besps.2019.6809.1013
Mohammad Ashraf Ahmad Ali; Hoda M. Moghazy; Ahmed Mostafa Mahmoud; Khaled Ahmed AbdelSater. "OREXIN-1-RECEPTOR BLOCKER, SB-334867 MAY AFFECT BODY WEIGHT AND PROTECT AGAINST HYPOGLYCEMIA INDUCED BY PARADOXICAL SLEEP DEPRIVATION IN ADULT MALE RATS". EKB Journal Management System, 39, 1, 2019, 91-101. doi: 10.21608/besps.2019.6809.1013
Ali, M., Moghazy, H., Mahmoud, A., AbdelSater, K. (2019). 'OREXIN-1-RECEPTOR BLOCKER, SB-334867 MAY AFFECT BODY WEIGHT AND PROTECT AGAINST HYPOGLYCEMIA INDUCED BY PARADOXICAL SLEEP DEPRIVATION IN ADULT MALE RATS', EKB Journal Management System, 39(1), pp. 91-101. doi: 10.21608/besps.2019.6809.1013
Ali, M., Moghazy, H., Mahmoud, A., AbdelSater, K. OREXIN-1-RECEPTOR BLOCKER, SB-334867 MAY AFFECT BODY WEIGHT AND PROTECT AGAINST HYPOGLYCEMIA INDUCED BY PARADOXICAL SLEEP DEPRIVATION IN ADULT MALE RATS. EKB Journal Management System, 2019; 39(1): 91-101. doi: 10.21608/besps.2019.6809.1013

OREXIN-1-RECEPTOR BLOCKER, SB-334867 MAY AFFECT BODY WEIGHT AND PROTECT AGAINST HYPOGLYCEMIA INDUCED BY PARADOXICAL SLEEP DEPRIVATION IN ADULT MALE RATS

Bulletin of Egyptian Society for Physiological Sciences
Article 7, Volume 39, Issue 1, June 2019, Page 91-101  XML PDF (828.45 K)
Document Type: Original Article
DOI: 10.21608/besps.2019.6809.1013
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Authors
Mohammad Ashraf Ahmad Ali email 1; Hoda M. Moghazy2; Ahmed Mostafa Mahmoud3; Khaled Ahmed AbdelSater4
1Department of Physiology, Faculty of medicine, Sohag University, Sohag, Egypt
2Medical Physiology Department, Faculty of Medicine, Sohag University, Egypt.
3physiology Department, Faculty of Medicine, Sohag University, Sohag, Egypt.
4department of Physiology, Faculty of medicine, Al-Azhar University, Assuit, Egypt
Abstract
Background: Sleep deprivation (SD) can affect health through its effects on many systems. Orexin is involved in regulation of many physiological functions including sleep. This can give an explanation and a way of protection against some hazards of SD.
Aim: To test the protective effect of orexin-1 receptor (OX1R) blocker, SB-334867 on changes in food intake, blood glucose level and insulin sensitivity caused by SD.
Method: 72 adult male rats arranged in 4 equal groups: control group, SD group, SD-OX1R blocked group & SD-DMSO group. The 3 SD groups are subjected to 8 days of paradoxical SD using the modified multiple platform method. The SD-OX1R blocked group was injected intraperitoneally daily with single dose of SB-334867 dissolved in 2 ml DMSO and diluted 1:1000 in saline (3 mg/kg/day). The SD-DMSO group was injected by DMSO alone. Food intake, body weight, blood fasting glucose & insulin levels were assessed and insulin resistance was calculated using HOMA-IR formula.
Results: The SD and SD-DMSO groups showed loss of weight inspite of increased food intake plus hypoglycemia with increased insulin sensitivity. The SD-OX1R blocked group showed no significant change in food intake but more drop in body weight plus delayed changes in fasting blood glucose and insulin sensitivity.
Conclusion: SD can affect health through its effect on food intake and induction of hypoglycemia. OX1R blocker, SB-334867 protects against the increase in food intake and delays increased insulin sensitivity and subsequent hypoglycemia. So, orexin most probably is a mechanism by which SD causes these changes.
Keywords
Sleep deprivation; orexin; insulin sensitivity
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