Cell-Free Therapy as A New Treatment Strategy in An Albino Rat Model of Cerebral Ischemia [Histological Studies] | ||||
| International Journal of Medical Arts | ||||
| Article 2, Volume 4, Issue 12, December 2022, Page 2849-2858 PDF (1.48 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ijma.2023.180341.1570 | ||||
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| Authors | ||||
Ahmed Eid Metwaly El-Naggar  1; Ashraf Mahmoud Mostafa2; Rania Abd-Elazim Galhom3; Ibrahim Mahrous Amr4; Mohamed Elsayed Mohamed Amer4; Mahmoud Fathy Ahmed 5
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| 1Department of Histology and Cytology, Damietta Faculty of Medicine, Al-Azhar Unviersity, Damietta, Egypt | ||||
| 2Department of Histology and Cytology, Faculty of Medicine, Al-Azhar University, Cairo, Egypt | ||||
| 3Department of Anatomy and Embryology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt | ||||
| 4Department of Histology and Cytology, Damietta Faculty of Medicine, Al-Azhar University, Damietta, Egypt | ||||
| 5Department of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt | ||||
| Abstract | ||||
| Background: The therapeutic efficacy of stem cell therapy in many diseased organs based greatly on their paracrine effect. The neural stem cells are thought to have a role in improving the neurological functional, recovery, and reduction of cerebral infarction caused by cerebral ischemia/ reperfusion [I/R] injury in rats. Objective: This study was conducted to look at the histological alterations in the rat cerebral cortex after ischemia-reperfusion [I/R] injury and to assess any potential neuroprotective effects of neural stem cells conditioned media [NSC-CM] on on those changes. Material and Methods: Thirty adult male albino rats weighing about 150 - 250 gm each were used in the present work. The animals were equally divided into 3 groups [10 rats each]. Group A was subdivided into negative control group, A1, [5 rats], not subjected to any intervention, and the sham-control group, A2 [5 rats], the animal of which were subjected to a midline cervical incision with exposure of both common carotid arteries followed by closure of the incision. Group B included rats subjected to brain [I/R] to induce transient global brain ischemia. Group C included rats subjected to I/R injury [as group B] and received 1.5 ml NSC-CM injected slowly through the tail vein at 3 h, 24 h, and 48 h after ischemia onset. Animals were anaesthetized using ether inhalation, the cerebrum was dissected and fixed in 10% neutral buffered formalin and specimens from them were taken and processed for examination by light microscopes. Results: NSC-CM significantly ameliorated neurological defects by reducing cerebral infarct volume, significant inhibition of cell apoptosis in the ischemic cerebral hemisphere Conclusion: NSC-CM might be an alternative and effective therapeutic intervention for ischemic stroke. | ||||
| Keywords | ||||
| Apoptosis; Cerebrum; I/R; NSC-CM; Rat | ||||
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