Chemical Characterization of Constituents Isolated from Hamelia patens and Investigating Its Cytotoxic Activity | ||||
| Egyptian Journal of Chemistry | ||||
| Article 10, Volume 62, Issue 9, September 2019, Page 1685-1697 PDF (1.33 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejchem.2019.10112.1667 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Maii Abdelnaby Ismail Maamoun   1; Salma Ahmed El Sawi2; Hemaia Mohamed Motawe1; Mostafa Ibrahim Fekry3; Mostafa Abdel Kawy3
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| 1Department of Pharmacognosy, National Research Centre, Cairo, Egypt | ||||
| 2Department of Pharmacognosy, National Research Centre | ||||
| 3Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo, Egypt | ||||
| Abstract | ||||
| SRB cytotoxicity assay was performed for different extracts of Hamelia patens Jacq. family Rubiaceae; crude flowers (CF), crude leaves (CL), chloroform (Chl.L) and methanol (Me.L) fractions from leaves against liver (HepG-2) and breast (MCF-7) human carcinoma cell lines. Results demonstrated potent cytotoxic action against HEPG-2 for CF, Chl.L and Me.L with IC50 = 47, 30 and 44.4 µg/mL, respectively. On the other hand, CF, CL, Chl.L and Me.L had exerted powerful effect against MCF-7 with IC50 = 23.8, 25.5, 17.7 and 64 µg/mL, respectively. Chromatographic investigation of Me.L , resulted in the isolation and spectroscopic identification of rutin (1), isoquercetin (2) and soyasaponin Bb (3), which is isolated for the first time from the plant leaves and was identified by UPLC/ITMS/MS analysis. Comparative study for using ordinary method versus green methods of extraction was also inspected. | ||||
| Keywords | ||||
| natural products; Hamelia patens; Cytotoxicity; Soyasaponin Bb; UPLCITMS | ||||
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