Assessment of IL-1B31 and IL RA gene Polymorphism in Immune Thrombocytopenia
diab, S., kamal, H., behairy, O., ELAMAWY, M., Shahin, E., Noureldin Abdelrahman, A. (2023). Assessment of IL-1B31 and IL RA gene Polymorphism in Immune Thrombocytopenia. EKB Journal Management System, 40(1), 298-309. doi: 10.21608/bmfj.2023.161067.1659
safeya mohamed diab; howaida mohamed kamal; ola galal behairy; MEDHAT ELAMAWY; Esraa Shahin; amira mohamed Noureldin Abdelrahman. "Assessment of IL-1B31 and IL RA gene Polymorphism in Immune Thrombocytopenia". EKB Journal Management System, 40, 1, 2023, 298-309. doi: 10.21608/bmfj.2023.161067.1659
diab, S., kamal, H., behairy, O., ELAMAWY, M., Shahin, E., Noureldin Abdelrahman, A. (2023). 'Assessment of IL-1B31 and IL RA gene Polymorphism in Immune Thrombocytopenia', EKB Journal Management System, 40(1), pp. 298-309. doi: 10.21608/bmfj.2023.161067.1659
diab, S., kamal, H., behairy, O., ELAMAWY, M., Shahin, E., Noureldin Abdelrahman, A. Assessment of IL-1B31 and IL RA gene Polymorphism in Immune Thrombocytopenia. EKB Journal Management System, 2023; 40(1): 298-309. doi: 10.21608/bmfj.2023.161067.1659

Assessment of IL-1B31 and IL RA gene Polymorphism in Immune Thrombocytopenia

Benha Medical Journal
Article 27, Volume 40, Issue 1, May and June 2023, Page 298-309  XML PDF (728.58 K)
Document Type: Original Article
DOI: 10.21608/bmfj.2023.161067.1659
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Authors
safeya mohamed diab1; howaida mohamed kamal1; ola galal behairy2; MEDHAT ELAMAWYorcid 3; Esraa Shahin email 4; amira mohamed Noureldin Abdelrahman5
1Clinical and Chemical pathology department, Faculty of medicine – Benha University
2Pediatrics department , Faculty of medicine – Benha University, Egypt
3Benha faculty of medicine , internal medicine department , Benha , EGYPT
4Clinical and Chemical pathology, Faculty of medicine – Benha University
5Clinical and chemical pathology Faculty of Medicine, Benha University
Abstract
Background
Primary immune thrombocytopenia (ITP) is one of the most common autoimmune diseases characterized by low platelet count resulting from increased platelet destruction and impaired platelet production. The pathophysiology of ITP remains understood, and the study of polymorphism in different cytokine coding genes is important to understating the pathophysiology of the disease. This study aimed to evaluate the association between IL1B-31 and IL1-RA gene polymorphism in ITP.
Patients and methods:
A case-control study was conducted on 50(30 children and 20 adults) ITP patients and 60 age and sex-matched healthy controls. Genotyping of IL1B-31 and IL1-RA gene polymorphism was performed using Restriction Fragment Length Polymorphism (RFLP-PCR) and detection of variable number tandem repeats.
Results :
The present study showed a significant association between IL1B-31 and IL1-RA gene polymorphism and ITP development. The mutant homozygous and heterozygous IL1B-31 and IL1-RA genotype and the mutant allele showed higher frequency compared to the control group and are associated with ITP susceptibility (odd ratio >1) , P value (
Keywords
Primary immune thrombocytopenia; IL-1B-31; IL-1Ra polymorphism; PCR-RFLP
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