Study of pathological and biochemical connection of IL-10 gene polymorphism and Rheumatoid Arthritis and Atopic Dermatitis in Egyptian patients admitted in local clinical setting
Atallah, R., Abdelrahman, A., Ahmed, A., Solyman, S. (2023). Study of pathological and biochemical connection of IL-10 gene polymorphism and Rheumatoid Arthritis and Atopic Dermatitis in Egyptian patients admitted in local clinical setting. EKB Journal Management System, 3(2), 40-49. doi: 10.21608/aijpms.2023.130076.1121
Rahma Atallah; Ali Abdelrahman; Ashraf Ahmed; Samar Solyman. "Study of pathological and biochemical connection of IL-10 gene polymorphism and Rheumatoid Arthritis and Atopic Dermatitis in Egyptian patients admitted in local clinical setting". EKB Journal Management System, 3, 2, 2023, 40-49. doi: 10.21608/aijpms.2023.130076.1121
Atallah, R., Abdelrahman, A., Ahmed, A., Solyman, S. (2023). 'Study of pathological and biochemical connection of IL-10 gene polymorphism and Rheumatoid Arthritis and Atopic Dermatitis in Egyptian patients admitted in local clinical setting', EKB Journal Management System, 3(2), pp. 40-49. doi: 10.21608/aijpms.2023.130076.1121
Atallah, R., Abdelrahman, A., Ahmed, A., Solyman, S. Study of pathological and biochemical connection of IL-10 gene polymorphism and Rheumatoid Arthritis and Atopic Dermatitis in Egyptian patients admitted in local clinical setting. EKB Journal Management System, 2023; 3(2): 40-49. doi: 10.21608/aijpms.2023.130076.1121

Study of pathological and biochemical connection of IL-10 gene polymorphism and Rheumatoid Arthritis and Atopic Dermatitis in Egyptian patients admitted in local clinical setting

Azhar International Journal of Pharmaceutical and Medical Sciences
Volume 3, Issue 2, June 2023, Page 40-49  XML PDF (465.01 K)
Document Type: Original research articles
DOI: 10.21608/aijpms.2023.130076.1121
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Authors
Rahma Atallah email orcid 1; Ali Abdelrahman1; Ashraf Ahmed2; Samar Solyman1
1Department of Microbiology and Immunology, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt
2Department of Dermatology and Andrology, Faculty of Medicine, Mansoura University, Mansoura, Egypt
Abstract
Rheumatoid arthritis (RA) is a chronic inflammation of the immune system that similarly affects the synovial joints. Atopic dermatitis (AD) is a chronic skin condition that is characterized by a complicated interaction of repeated exposures, host factors, and hereditary factors. Interleukin 10 (IL-10) is an immune-regulatory cytokine that is commonly utilized in RA to reduce the inflammatory response. An allelic variation in the IL-10 gene's promoter region may play a role in autoantibody production control. This study aims to identify Polymorphisms in the IL-10 gene in RA and AD patients and sequence the IL-10 gene fragment to elucidate the polymorphism (IL10-1082A/G) and determine the possible relationship between IL10 gene polymorphism and pathogenesis of RA and AD. Twenty-five blood samples were collected from RA Egyptian patients / 17 healthy control samples and 19 blood samples were collected from AD Egyptian patients / 12 healthy control. DNA has been extracted from blood samples and subjected to Restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) test for the determination of IL-10 genotype. PCR products were sent for sequencing. Genotyping revealed that both the patient and control groups had the same IL-10 allele; sequencing results showed silent mutation with no effect in the amino acid sequence. Our results showed that there is no association between IL10-1082 A/G single nucleotide polymorphism and RA or AD pathogenesis. Environmental factors may have more effect on the pathogenesis of RA and AD.
Keywords
Genotyping; Inflammatory disorders; RFLP PCR; Sequencing; pathogenesis
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