Immunological And Genotyping Study of Toxoplasma Gondi and Its Relationship with Toll Like Receptor 4 “TLR4” Polymorphism in Aborted Women
Alkardhi, I., Masmoudi, H., Muhammed, H., Sellami, H. (2023). Immunological And Genotyping Study of Toxoplasma Gondi and Its Relationship with Toll Like Receptor 4 “TLR4” Polymorphism in Aborted Women. EKB Journal Management System, 90(2), 3338-3345. doi: 10.21608/ejhm.2023.291354
Ihsan K. A. Alkardhi; Hatem Masmoudi; Hayder A. Muhammed; Hayet Sellami. "Immunological And Genotyping Study of Toxoplasma Gondi and Its Relationship with Toll Like Receptor 4 “TLR4” Polymorphism in Aborted Women". EKB Journal Management System, 90, 2, 2023, 3338-3345. doi: 10.21608/ejhm.2023.291354
Alkardhi, I., Masmoudi, H., Muhammed, H., Sellami, H. (2023). 'Immunological And Genotyping Study of Toxoplasma Gondi and Its Relationship with Toll Like Receptor 4 “TLR4” Polymorphism in Aborted Women', EKB Journal Management System, 90(2), pp. 3338-3345. doi: 10.21608/ejhm.2023.291354
Alkardhi, I., Masmoudi, H., Muhammed, H., Sellami, H. Immunological And Genotyping Study of Toxoplasma Gondi and Its Relationship with Toll Like Receptor 4 “TLR4” Polymorphism in Aborted Women. EKB Journal Management System, 2023; 90(2): 3338-3345. doi: 10.21608/ejhm.2023.291354

Immunological And Genotyping Study of Toxoplasma Gondi and Its Relationship with Toll Like Receptor 4 “TLR4” Polymorphism in Aborted Women

The Egyptian Journal of Hospital Medicine
Article 206, Volume 90, Issue 2, January 2023, Page 3338-3345  XML PDF (869.25 K)
Document Type: Original Article
DOI: 10.21608/ejhm.2023.291354
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Authors
Ihsan K. A. Alkardhi email ; Hatem Masmoudi; Hayder A. Muhammed; Hayet Sellami
Abstract
Background:  Toxoplasma gondii (T. gondii) causes toxoplasmosis, a dangerous and prevalent disease. Toll-like receptors (TLRs) are the best-studied pattern recognition receptors in mammals.
Objective: The current study was conducted at Al-Diwaniya Maternity and Children Teaching Hospital, Diwaniyah city, Iraq, from December 2020 to August 2021. Methods: Blood samples and placenta tissue pieces were collected prospectively from 30 patients newly diagnosed with toxoplasmosis and 64 healthy controls. Women in both groups underwent a spontaneous abortion. A human Toll-Like Receptor 4 (TLR4) ELISA kit was used to measure TLR4 levels and blood DNA extraction. ARMS-PCR was adopted to analyze the polymorphism for TLR4 Asp299Gly, and SAG3 marker was used to analyze the genotype of Toxoplasma strains.
Results: The frequency distribution of the A allele and G allele was statistically non-significant between the patients and the control group. The polymorphism analysis of the Asp299Gly SNP in the TLR4 gene showed abortion had no significant association with toxoplasma infection (P> 0.05). Genotype II was more prevalent in aborted Iraqi women (22/30) than in control. The obtained result revealed that the concentration of TLR4 in serum women infected with type I Toxoplasma was significantly higher (P< 0.05) in the AA genotype of TLR4 Asp299Gly ( mean 1328 pg/ml, SD: 266.1pg/ml ), compared to AG genotype (mean; 923.6 pg/ml, SD: 27.2 pg/ml).
Discussion: These findings highlighted the significance of TLR molecules in Toxoplasma gondii infection and the need for close collaboration between practitioners and to lessen the illness burden of toxoplasmosis.
 
Keywords
Toxoplasma gondii; Sag3; TLR4; Asp299Gly Polymorphism; ARMS-PCR
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