Isolation and Structure Elucidation of Aromatic Polyketides from Marine Actinomycete with Antibiofilm Activity against Staphylococcus aureus and Escherichia coli
mahmoud, A., Ali, S., Ebrahim, H., Hebishy, A., Essawy, E., Abdelfattah, M. (2023). Isolation and Structure Elucidation of Aromatic Polyketides from Marine Actinomycete with Antibiofilm Activity against Staphylococcus aureus and Escherichia coli. EKB Journal Management System, 66(12), 303-311. doi: 10.21608/ejchem.2023.183616.7451
AYa Ramadan mahmoud; Sally A Ali; Hasan Y Ebrahim; Ali Hebishy; Ehab A. Essawy; Mohamed saleh Abdelfattah. "Isolation and Structure Elucidation of Aromatic Polyketides from Marine Actinomycete with Antibiofilm Activity against Staphylococcus aureus and Escherichia coli". EKB Journal Management System, 66, 12, 2023, 303-311. doi: 10.21608/ejchem.2023.183616.7451
mahmoud, A., Ali, S., Ebrahim, H., Hebishy, A., Essawy, E., Abdelfattah, M. (2023). 'Isolation and Structure Elucidation of Aromatic Polyketides from Marine Actinomycete with Antibiofilm Activity against Staphylococcus aureus and Escherichia coli', EKB Journal Management System, 66(12), pp. 303-311. doi: 10.21608/ejchem.2023.183616.7451
mahmoud, A., Ali, S., Ebrahim, H., Hebishy, A., Essawy, E., Abdelfattah, M. Isolation and Structure Elucidation of Aromatic Polyketides from Marine Actinomycete with Antibiofilm Activity against Staphylococcus aureus and Escherichia coli. EKB Journal Management System, 2023; 66(12): 303-311. doi: 10.21608/ejchem.2023.183616.7451

Isolation and Structure Elucidation of Aromatic Polyketides from Marine Actinomycete with Antibiofilm Activity against Staphylococcus aureus and Escherichia coli

Egyptian Journal of Chemistry
Volume 66, Issue 12, December 2023, Page 303-311  XML PDF (663.28 K)
Document Type: Original Article
DOI: 10.21608/ejchem.2023.183616.7451
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Authors
AYa Ramadan mahmoud1; Sally A Ali2; Hasan Y Ebrahim3; Ali Hebishy4; Ehab A. Essawy5; Mohamed saleh Abdelfattah email orcid 6
1Faculty of science, Helwan university
2Botany and Microbiology Department, Faculty of Science, Helwan University, Cairo 11795, Egypt
3Faculty of Pharmacy, Helwan University, Ain Helwan, Cairo 11795, Egypt.
4Faculty of Science, Helwan University
5Chemistry Department, Faculty of Science, Helwan University, Ain Helwan, Cairo, Egypt.
6Natural Products Research Unit (NPRU), Chemistry Department, Faculty of Science, Helwan University, Egypt
Abstract
Three pigmented secondary metabolites tetracenomycin D (1), resistomycin (2), and resistoflavin (3) were isolated from Streptomyces sp. EG1 and their antibiofilm activities were evaluated against Staphylococcus aureus and Escherichia coli. The isolated compounds were purified by different chromatographic methods, including Silica gel and Sephadex LH-20, and their chemical structures were elucidated by mass and NMR spectroscopy. Tetracenomycin D (1) inhibited 75% of both pathogens from creating biofilm at 400 µg/ml, whereas resistomycin (2), and resistoflavin (3) inhibited biofilm by more than 80% at the same concentration. The light microscopic investigation revealed that compounds 1-3 inhibit the formation of biofilms and the adhesion of bacterial cells to the surface of glass pieces. As observed by confocal laser scanning microscopy (CLSM), pigmented metabolites 1-3 disrupt the 3D architecture of biofilm-forming bacteria. Molecular docking was used to find out how compounds 1–3 bind to the active sites of the biofilm-forming proteins ClfB and CsgG in S. aureus and E. coli, respectively.
Keywords
actinomycetes; antibiofilm; light microscope; confocal laser scanning microscope; ClfB; CsgG; molecular docking
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