Diisooctyl Phthalate as A Secondary Metabolite from Actinomycete Inhabit Animal’s Dung with Promising Antimicrobial Activity
Abou El-Enain, I., Zeatar, A., Zayed, A., Elkhawaga, M., Mahmoud, Y. (2023). Diisooctyl Phthalate as A Secondary Metabolite from Actinomycete Inhabit Animal’s Dung with Promising Antimicrobial Activity. EKB Journal Management System, 66(12), 261-277. doi: 10.21608/ejchem.2023.172600.7412
Inas Abou El-Enain; Amal Zeatar; Ahmed Zayed; Maie Elkhawaga; Yehia Mahmoud. "Diisooctyl Phthalate as A Secondary Metabolite from Actinomycete Inhabit Animal’s Dung with Promising Antimicrobial Activity". EKB Journal Management System, 66, 12, 2023, 261-277. doi: 10.21608/ejchem.2023.172600.7412
Abou El-Enain, I., Zeatar, A., Zayed, A., Elkhawaga, M., Mahmoud, Y. (2023). 'Diisooctyl Phthalate as A Secondary Metabolite from Actinomycete Inhabit Animal’s Dung with Promising Antimicrobial Activity', EKB Journal Management System, 66(12), pp. 261-277. doi: 10.21608/ejchem.2023.172600.7412
Abou El-Enain, I., Zeatar, A., Zayed, A., Elkhawaga, M., Mahmoud, Y. Diisooctyl Phthalate as A Secondary Metabolite from Actinomycete Inhabit Animal’s Dung with Promising Antimicrobial Activity. EKB Journal Management System, 2023; 66(12): 261-277. doi: 10.21608/ejchem.2023.172600.7412

Diisooctyl Phthalate as A Secondary Metabolite from Actinomycete Inhabit Animal’s Dung with Promising Antimicrobial Activity

Egyptian Journal of Chemistry
Volume 66, Issue 12, December 2023, Page 261-277  XML PDF (828.07 K)
Document Type: Original Article
DOI: 10.21608/ejchem.2023.172600.7412
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Authors
Inas Abou El-Enain1; Amal Zeatar1; Ahmed Zayed email orcid 2; Maie Elkhawaga1; Yehia Mahmoud3
1Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Girls Branch, Cairo, Egypt
2Pharmacognosy Department, Tanta University, Tanta, Egypt
3Botany Department, Faculty of Science, Tanta University, Tanta, 31527, Egypt
Abstract
In the present work, actinomycete isolates with potential antibacterial activity were sought from animal manure. Twenty-two actinomycete isolates were isolated and nominated on the basis of their cultural properties on starch nitrate agar medium. The antibacterial activity of these isolates against a variety of bacterial and fungal species was examined. Actinomycete isolate A3 showed the highest antibacterial activity among those examined. The morphological, physiological, biochemical, and molecular characteristics of isolate A3 indicate that it belongs to the strain Streptomyces marokkonensis LMG 23016. The optimal conditions for development and antibacterial activity were submerged cultivation at 30 degrees Celsius and a pH of 7 for seven days. The optimal carbon and nitrogen sources are starch and potassium nitrate, respectively, and 1 % (w/v) sodium chloride. The antimicrobial metabolites of isolate A3 were extracted and analyzed using thin-layer chromatography (TLC), which revealed a solitary spot. UV, IR, HPLC, and GC/MS analysis confirmed diisooctyl phthalate (1,2-benzene dicarboxylic acid, diisooctyl ester) to be the major component. The results of the minimum inhibitory concentration and minimum bactericidal concentration tests on the S. marokkonensis strain LMG 23016 A3 extract demonstrated a broad-spectrum action against bacteria, thereby creating new opportunities for the development of potent antibiotic candidates.
Keywords
Animals’ dung actinomycetes; Diisooctyl phthalate; Streptomyces marokkonensis
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