Prophylactic Effects of DL-β Hydroxybutyrate against Hepatic Cellular Senescence Induced by D-galactose or γ-irradiation via Partial Modulation of Antioxidants and Trace Elements in Male Rats | ||||
| Egyptian Journal of Radiation Sciences and Applications | ||||
| Volume 35, Issue 1, December 2022, Page 113-128 PDF (1.16 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejrsa.2023.187973.1148 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Mahmoud E. Habieb1; Marwa A. Mohamed  1; Doaa M. El Gamal2; Asrar M. Hawas1; Tarek M. Mohamed 2
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| 1Drug Radiation Research Department, National Centre for Radiation Research and Technology (NCRRT), Atomic Energy Authority, P.O. Box; 29 Nasr city, Cairo, Egypt | ||||
| 2Biochemistry Division, Chemistry Department, Faculty of Science, Tanta University, Tanta, Egypt | ||||
| Abstract | ||||
| Background: aging of cells is a normal feature but, it is important to delay this process and improve the life style. Aim: this work aimed to investigate the possible potential inhibitory effect of DL-β-hydroxybutyrate (βOHB) against hepatic cellular senescence induced by D-galactose or γ-irradiation in rats based on antioxidant and certain trace elements estimation in the liver. Methods: Six groups of male rats were used as the control, irradiated group (acute dose 5 Gy, 2 weeks), D-galactose (150 mg/kg b.wt, 6 weeks), βOHB (72.8 mg/kg b.wt, 14 days), γ-irradiation plus βOHB, and D-galactose plus βOHB. Results: neither βOHB nor D-galactose administration had a significant effect relative to the normal control value on Hb, MCV, and RBC’s. βOHB along with D-galactose or γ-irradiation significantly increased G6PD activity compared with control group. In D-galactose, or γ-irradiation groups, liver MDA levels and SOD activity were significantly increased. Meanwhile, NO and GSH levels were significantly increased in the γ-irradiation group relative to normal control levels. The findings showed that βOHB alleviated hematological, antioxidant alterations and modulated the change in Cu, Fe, and Zn elements in D-galactose or γ-irradiation group. The results concur well with histological alteration in our previous findings. Conclusion: these findings highlight a role for βOHB as a potent protective agent against hepatic cellular senescence associated liver injury through enhancing RBC’s G6PD activity, reduction of oxidative stress and partial modulation of trace elements. | ||||
| Keywords | ||||
| D-galactose; Gamma irradiation; Oxidative stress; β-hydroxybutyrate; Trace elements | ||||
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