Histological Evaluation of the Emerging Role of Adipose Stem Cells-Derived Exosomes in Cutaneous Wound Healing in Albino Rats | ||||
| Egyptian Journal of Histology | ||||
| Article 8, Volume 41, Issue 4, December 2018, Page 459-472 PDF (2.23 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejh.2018.4507.1015 | ||||
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| Authors | ||||
Dalia Ibrahim Ismail   ; Alshaymaa Gamal Aboulkhair
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| Histology Department, Faculty of Medicine, Cairo University, Cairo, Egypt | ||||
| Abstract | ||||
| Prolonged healing and scar formation are two major challenges in treating wounds, resulting in physical, mental and socioeconomic burden to the affected subjects. Exosomes (Exos) are naturally secreted vesicles playing a vital role in the transfer of RNA to neighboring or distant recipient cells and there are numerous on-going studies on their role of in tissue regeneration and wound healing. Aim of the work: Evaluation of the efficiency of Exos derived from adipose stem cells on cutaneous wound healing in adult male albino rats. Materials and Methods: Thirty-four rats were divided into three groups; Group I: control. Group II: a dorsal cut wound (1.5 × 1.5 cm) was done to each rat. Group III: the same wound was done and rats were injected with 200μ PKH26-labeled Exos intravenously. Rats were sacrificed after 21 days. Skin sections were subjected to hematoxylin and eosin (HandE), Mallory's trichrome and orcein stains, alpha smooth muscle actin (α-SMA) and Ki67 immunohistochemical stains. Results: Skin sections from group II showed scar formation, lost epidermal layers and hair follicles. There was significant increase in collagen fibers and α-SMA immunoreaction, with significant decrease in elastic fibers and Ki67 immunoexpression compared to the control. In group III, the skin revealed almost normal histology, significant decrease in collagen fibers and α-SMA, with significant increase in elastic fibers and Ki67 immunoreaction compared to group II. Conclusion: Exos injection has demonstrated a beneficial effect on cutaneous wound healing and could prevent scar formation in adult male albino rats. | ||||
| Keywords | ||||
| Cutaneous Scar; exosomes; myofibroblast; skin Wound; Wound healing | ||||
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