Possible protective effect of pomegranate peel powder on lung injury induced by skeletal muscle ischemia-reperfusion | ||||
| Egyptian Journal of Histology | ||||
| Article 14, Volume 41, Issue 4, December 2018, Page 546-558 PDF (2.67 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejh.2019.28766 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Rasha Salama,  ; Manar Ali Faried
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| Department of Anatomy and Embryology, Faculty of Medicine, Menoufia University, Egypt | ||||
| Abstract | ||||
| Introduction: Multi-organ failure with life-threatening sequel occurs in a variety of surgical practices as a result of ischemia-reperfusion (I/R) injury. Aim of work: In this study, more light is thrown on the histological lung changes induced by skeletal muscle I/R with reference to their underlying mechanisms and to determine, for the first time, the protective role of pomegranate peel powder on the lung injury induced by skeletal muscle I/R. Materials and Methods: Thirty adult male albino rats were divided equally into three groups: sham control group, I/R group and I/R group pretreated with pomegranate peel powder (500 mg/kg dissolved in distilled water twice daily orally for 10 days). At the end of the experiment, blood samples were obtained to assess serum malondialdehyde (MDA) then lung specimens were processed for routine histological examination and detection of collagen deposition. Myeloperoxidase (MPO), a marker of neutrophils infiltration, transforming growth factor-β (TGF-β), an inflammatory and pro-fibrotic mediator and Bcl2, an anti-apoptotic marker, were detected immunohistochemically. Results: Loss of general architecture of the lung tissues, massive inflammatory infiltrate, emphysema, edema, congested blood vessels, thickened inter-alveolar septa, destruction of some alveoli, disorganization and focal sloughing of the bronchial epithelium in addition to increased collagen deposition were detected in I/R group. Oxidative stress in the form of significant increase of MDA level in addition to up-regulation of both MPO and TGF-β and down-regulation of Bcl2 were implicated in the pathogenesis of acute lung injury induced by skeletal muscle I/R. Pomegranate peel powder significantly ameliorated these damaging effects through its antioxidant, anti-inflammatory, anti-fibrotic and anti-apoptotic effects. Conclusion: Pretreatment of rats with pomegranate peel protected the lungs against skeletal muscle I/R injury. | ||||
| Keywords | ||||
| Ischemia-reperfusion; lung; Pomegranate peel | ||||
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