A histological Study Comparing the Possible Protective Mechanisms of Oxytocin and Vitamin E on ischemia reperfusion injury in Rat skeletal muscle
Nada, S., El-Deeb, D., AboulKhair, A., Hosny, S. (2018). A histological Study Comparing the Possible Protective Mechanisms of Oxytocin and Vitamin E on ischemia reperfusion injury in Rat skeletal muscle. EKB Journal Management System, 41(4), 570-581. doi: 10.21608/ejh.2018.5067.1021
Samir Mahmoud Nada; Dalia Fathy El-Deeb; Alshaymaa Gamal AboulKhair; Sara Adel Adel Hosny. "A histological Study Comparing the Possible Protective Mechanisms of Oxytocin and Vitamin E on ischemia reperfusion injury in Rat skeletal muscle". EKB Journal Management System, 41, 4, 2018, 570-581. doi: 10.21608/ejh.2018.5067.1021
Nada, S., El-Deeb, D., AboulKhair, A., Hosny, S. (2018). 'A histological Study Comparing the Possible Protective Mechanisms of Oxytocin and Vitamin E on ischemia reperfusion injury in Rat skeletal muscle', EKB Journal Management System, 41(4), pp. 570-581. doi: 10.21608/ejh.2018.5067.1021
Nada, S., El-Deeb, D., AboulKhair, A., Hosny, S. A histological Study Comparing the Possible Protective Mechanisms of Oxytocin and Vitamin E on ischemia reperfusion injury in Rat skeletal muscle. EKB Journal Management System, 2018; 41(4): 570-581. doi: 10.21608/ejh.2018.5067.1021

A histological Study Comparing the Possible Protective Mechanisms of Oxytocin and Vitamin E on ischemia reperfusion injury in Rat skeletal muscle

Egyptian Journal of Histology
Article 16, Volume 41, Issue 4, December 2018, Page 570-581  XML PDF (2.3 MB)
Document Type: Original Article
DOI: 10.21608/ejh.2018.5067.1021
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Authors
Samir Mahmoud Nada1; Dalia Fathy El-Deeb1; Alshaymaa Gamal AboulKhair1; Sara Adel Adel Hosny email orcid 2
1Histology Department, Faculty of Medicine, Cairo University, Cairo, Egypt
2Histology department, faculty of medicine, cairo university
Abstract
Background: Ischemia-reperfusion (IR) injury in skeletal muscle is a pathophysiology which affect quality of life.
Aim of Work: To compare the possible protective mechanisms by which oxytocin and Vitamin E may exert on skeletal muscle IR injury in rat.  Materials and Methods: 28 male rats were divided into: Control (Group A) of 10 rats and IR group (Group B) of 18 rats. Control group underwent the same surgical steps as IR group but without clamping the femoral artery followed by the injection of one of the following [saline (AI), oxytocin (AII) or vitamin E (AIII)]. In the IR group, lower limb ischemia was induced by clamping the femoral artery. After 4h of ischemia, the clamp was removed to allow reperfusion coinciding with the injection of one of the following [saline (BI), Oxytocin (0.5μg/kg) (BII) and Vitamin E (10mg/kg) (BIII)]. After 2 hours of reperfusion, Blood samples were drawn from the femoral vein to measure glutathione (GSH) and malondialdehyde (MDA). Gastrocnemius muscles were obtained for evaluation by light and electron microscopes. Cytochrome C, Myogenin immunohistochemistry and statistical analysis were applied.
Results: A significant increase in GSH and significant decrease in MDA were reported in BII and BIII as compared to BI. Mean area % of cytochrome C in BII and BIII showed a significant decrease compared to BI. Mean number of myogenin immunopositive cells in BII was significantly higher compared to BI. Muscular damage was proved by light and electron microscopic findings in BI. While, BII and BIII encountered evident protection from muscular damage induced by IR injury.  Conclusion: Oxytocin exerts weak protective antioxidant effect against IR injury. However, it activates stem cells to regenerate the damaged muscle.  Vitamin E was better antioxidant which can react as early as 1 hour in the protection of the skeletal muscle against IR injury.
Keywords
Ischemia reperfusion; myogenin; oxytocin; skeletal muscle; Vitamin E
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