CORRELATION BETWEEN FERTILITY AND LEVELS OFPROTEIN IN SEMINAL PLASMA OF EGYPTIANBUFFALO BULLS | ||||
| Journal of Sustainable Agricultural Sciences | ||||
| Article 4, Volume 42, Issue 3, September 2016, Page 82-89 PDF (231.53 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jsas.2016.2933 | ||||
 View on SCiNiTO | ||||
| Abstract | ||||
| The Objective of this study was to measure the protein profiles of seminalplasma in Egyptian buffalo bulls semen and to examine their correlation withsemen characteristics of buffalo bulls in Mehalet Mousa . Semen of 20 buffalobulls ( dividedinto high and low fertile bull, 10 for each group,according to records of insemination) were collected twice weekly for 12weeks by artificial vagina . Semen characteristics (ejaculate volume, motility ,abnormalities, viability and sperm cell concentration were recorded . Seminalplasma was seperated by centrifugation , treated with cold ethanol and then underwent SDS-polyacrylamide gel electro phoresis ( PAGE) . Fourteenprotein bands were Identified on the gel of these protein band . 25 KDa wassignificantly correlated with all semen parameters studied in high fertile group, while 63 KDa was significantly correlated with sperm motility and spermabnormalities in low fertile Group . | ||||
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