Gold Nanoparticle Biosynthesis-Mediated Acinetobacter Ƅɑumannii as a Cytotoxic and Apoptosis Inducer in Prostate Cancer Cell Lines | ||||
| The Egyptian Journal of Hospital Medicine | ||||
| Article 19, Volume 91, Issue 1, April 2023, Page 3885-3895 PDF (853.1 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejhm.2023.293476 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Intesar H. Al-Abdeli  ; Esam J. Al-kalifawi
| ||||
| Abstract | ||||
| Background: Gold nanoparticles (AuNPs), unlike chemotherapy, may destroy cancer cells without harming normal cells. Objective: For the first time, Acinetobacter (A.) baumannii-biosynthesized AuNPs (Ab-AuNPs) were evaluated for anticancer properties. Materials and methods: The Ab-AuNPs were characterized by UV-visible spectroscopy, TEM, FE-SEM, AFM, XRD analysis, and FTIR. The MTT assay was used to determine the antiproliferative efficacy of the Ab-AuNPs at different concentrations against prostate cancer (PC3) and human breast normal cell line (MCF-10). Potential apoptotic-mediated cell death was investigated using acridine orange and propidium iodide staining and mitochondrial membrane potential (MMP). Results: Biosynthesized Ab-AuNP had a zeta potential below 30 mV at -22 mV, suggesting particles stability with no aggregation. The average size was estimated at 66 nm (range 20-90 nm). Ab-AuNPs inhibited PC3 cell proliferation dose-dependently, with no adverse effect on MCF-10 cells. The mean lethal dose (IC50) was 13.72 µg/mL, and the maximal inhibitory concentration was 25.50 mg/mL (57.33% and 80.67%, respectively). Examining PC3 cancer cells treated with Ab-AuNPs at IC50 for 24 hours by flow cytometry revealed an increase in apoptosis that resulted in the disruption of the membrane and vacuoles of the lysosomes, which corresponded with the depletion of MMP. This was in contrast to MCF-10 AuNPs-treated cells, which showed no adverse effects. Conclusions: Producing gold nanoparticles in an A. baumannii broth culture is simple, inexpensive, and non-toxic to healthy cells. They showed anticancer effects by inducing apoptosis, making them a potential effective anticancer agent. | ||||
| Keywords | ||||
| Gold nanoparticles; Acinetobacter baumannii; Anti-cancer activity; Apoptosis activity | ||||
|
Statistics Article View: 213 PDF Download: 331 |
||||
