Detection of Rabies Virus and Its Antibodies Using Staphylococcus aureus Protein A | ||||
| Zagazig Veterinary Journal | ||||
| Article 8, Volume 43, Issue 1, March 2015, Page 87-93 PDF (458.92 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/zvjz.2015.29350 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Hemmat S A; A M Albehwar; M B Shendy | ||||
| Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo, P. O. Box : 131 | ||||
| Abstract | ||||
| Staph protein-A conjugated with fluorescein isothiocyanate and horseradish peroxidase were prepared and used in indirect fluorescent antibody technique (IFAT) and enzyme linked immunosorbent assay (ELISA), respectively. In addition, serum neutralization test (SNT) was used to detect post-vaccination rabies antibodies in sera of 30 vaccinated dogs and 20 vaccinated cats. Unconjugated staph protein -A was used of agglutination test (AT). All the form mentioned and applied methods for detection of the incidence of anti-rabies antibodies in animal sera even with the use of non- specific anti-species (staph protein-A). FAT, AT and ELISA were found to be specific; sensitive and rapid tests required for rapid detection of rabies antibodies and virus in infected cell culture and mice brain. Regarding the sensitivity and rapidity of these tests, FAT comes first followed by ELISA and SNT then AT. It concluded that staph protein-A either unconjugated or conjugated as non-specific anti-species can be used for rapid detection of rabies antibodies in the sera of vaccinated dogs and cats. | ||||
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