Immune response of Enterobacter aerogenes ghost vaccine in mice
Mohammed, F., Keshta, A., Hassan, M., Ghoneim, H., Amara, A., Sheweita, S. (2023). Immune response of Enterobacter aerogenes ghost vaccine in mice. EKB Journal Management System, 19(1), 1-11. doi: 10.21608/blj.2023.294038
Faten Zahran Mohammed; Akaber Tarek Keshta; Mahmoud Ayman Hassan; Hossam Ghoneim; Amro A Amara; Salah Ahmed Sheweita. "Immune response of Enterobacter aerogenes ghost vaccine in mice". EKB Journal Management System, 19, 1, 2023, 1-11. doi: 10.21608/blj.2023.294038
Mohammed, F., Keshta, A., Hassan, M., Ghoneim, H., Amara, A., Sheweita, S. (2023). 'Immune response of Enterobacter aerogenes ghost vaccine in mice', EKB Journal Management System, 19(1), pp. 1-11. doi: 10.21608/blj.2023.294038
Mohammed, F., Keshta, A., Hassan, M., Ghoneim, H., Amara, A., Sheweita, S. Immune response of Enterobacter aerogenes ghost vaccine in mice. EKB Journal Management System, 2023; 19(1): 1-11. doi: 10.21608/blj.2023.294038

Immune response of Enterobacter aerogenes ghost vaccine in mice

Biochemistry Letters
Volume 19, Issue 1, 2023, Page 1-11  XML PDF (701.75 K)
Document Type: Original Article
DOI: 10.21608/blj.2023.294038
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Authors
Faten Zahran Mohammed1; Akaber Tarek Keshta1; Mahmoud Ayman Hassan1; Hossam Ghoneim2; Amro A Amara3; Salah Ahmed Sheweita4, 5
1Department of Chemistry (Biochemistry Division, Faculty of Science, Zagazig University, Egypt
2Department of Immunology and Allergy, Medical Research Institute, Alexandria University, Egypt
3Department of Protein Research, City of Scientific Research and Technological Applications, Alexandria, Egypt
4Department of Clinical Biochemistry, Faculty of Medicine, King Khalid University, Abha, KSA.
5Department of Biotechnology, Institute of Graduate Studies and Research, Alexandria University, Egypt
Abstract
Background: The emergence of multidrug-resistant Enterobacter aerogenes has become a public health concern. E. aerogenes is a common cause of hospital-acquired infections such as pneumonia and urinary tract infections. Aim: This study focused on the use of E. aerogenes ghosts as a potential vaccine against the opportunistic pathogen E. aerogenes. Methods: This study used a cost-effective method to generate the E. aerogenes ghosts using certain chemicals. The safety and effectiveness of the ghosts were evaluated in a mouse model by administering three doses over a 14-day period. The mice were then challenged with a live E. aerogenes strain. Results: The results of the cytokine assays showed that vaccinated mice produced higher amounts of IL-10 and TNF-α than did control saline mice, indicating a good immune response. Additionally, the results of the liver function tests indicated that there was a decrease in the activities of ALT and AST in the vaccinated mice as compared to the non-vaccinated mice and low levels of TP and Alb in non-vaccinated mice, suggesting a reduced liver injury and inflammation in vaccinated mice. Conclusion: Overall, the study suggests that E. aerogenes ghosts may be a promising candidate for use as a vaccine against E. aerogenes infection.
Keywords
Bacterial ghost; Enterobacter aerogenes; Vaccine; Immune response; Cytokines
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