Comparison of replication competence between two highly pathogenic avian influenza viruses H5N1 and H5N8 in vitro | |||||||||||||||||||||||||||||||||||||||||||||||||
Egyptian Journal of Animal Health | |||||||||||||||||||||||||||||||||||||||||||||||||
Volume 3, Issue 2, April 2023, Page 44-51 PDF (1.24 MB) | |||||||||||||||||||||||||||||||||||||||||||||||||
Document Type: Original researches | |||||||||||||||||||||||||||||||||||||||||||||||||
DOI: 10.21608/ejah.2023.294051 | |||||||||||||||||||||||||||||||||||||||||||||||||
View on SCiNiTO | |||||||||||||||||||||||||||||||||||||||||||||||||
Abstract | |||||||||||||||||||||||||||||||||||||||||||||||||
Mixed infections with respiratory viruses have become frequent at poultry farm business in Egypt. The pandemic of highly pathogenic avian influenza virus (HPAIV) H5N1 (clade 2.2.1.2) has been spreading through the country since 2006. In 2016, the Spread of HPAIV H5N8 (clade 2.3.4.4b) to Egypt via wild birds into commercial poultry flocks made overburden on the economic situation of poultry business. The surveillance efforts conducted by the governmental veterinary authority GOVS showed dominating infection of H5N8 with rare spread of H5N1 in poultry market sector. Thus, the current study try to figure out whether a replication competence exhibited between the two highly pathogenic avian influenza viruses HPAIV H5N1 and H5N8 through designation an in-vitro induced infection of H5N8 and H5N1 in specific pathogen free embryonated chicken eggs (SPF- ECE). A concentration of 103 EID50 of HPAIV H5N1 and H5N8 were either sequentially or simultaneously inoculated into the allantoic cavity of SPF-ECE at 48 h of incubation, followed by the collection of allantoic fluid. A quantitative reverse transcription real-time polymerase chain reaction was used to determine the quantitative replication of viral RNA copies of neuraminidase gene of both AIV strains. A replication competence was observed according to which viral strain firstly inoculated. Whereas, H5N1 RNA titers was reduced by ≥4log10 when H5N8 firstly inoculated. Vice versa H5N8 RNA titers were reduced by ≥4log10 when H5N1 firstly inoculated. The interference impact of H5N1 was more powerful than that of H5N8 when simultaneous inoculation of both viruses was applied. In conclusion, quantitative evaluation of mixed inoculation of AIV strains exhibited replication competence of the first virus inoculation on the account of the succeeding one regardless of viral strain. However, H5N8 showed lower replication intensity according to the neuraminidase gene evaluation when both H5N1 and H5N8 were simultaneously inoculated in SPF ECE. | |||||||||||||||||||||||||||||||||||||||||||||||||
Keywords | |||||||||||||||||||||||||||||||||||||||||||||||||
avian influenza virus; H5N1; H5N8; quantitative real-time polymerase chain reaction; viral interference | |||||||||||||||||||||||||||||||||||||||||||||||||
Full Text | |||||||||||||||||||||||||||||||||||||||||||||||||
Comparison of replication competence between two highly pathogenic avian influenza viruses H5N1 and H5N8 in vitro
*Reference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Agriculture Research Center, Giza 12618, Egypt
Mixed infections with respiratory viruses have become frequent at poultry farm business in Egypt. The pandemic of highly pathogenic avian influenza virus (HPAIV) H5N1 (clade 2.2.1.2) has been spreading through the country since 2006. In 2016, the Spread of HPAIV H5N8 (clade 2.3.4.4b) to Egypt via wild birds into commercial poultry flocks made overburden on the economic situation of poultry business. The surveillance efforts conducted by the governmental veterinary authority GOVS showed dominating infection of H5N8 with rare spread of H5N1 in poultry market sector. Thus, the current study try to figure out whether a replication competence exhibited between the two highly pathogenic avian influenza viruses HPAIV H5N1 and H5N8 through designation an in-vitro induced infection of H5N8 and H5N1 in specific pathogen free embryonated chicken eggs (SPF- ECE). A concentration of 103 EID50 of HPAIV H5N1 and H5N8 were either sequentially or simultaneously inoculated into the allantoic cavity of SPF-ECE at 48 h of incubation, followed by the collection of allantoic fluid. A quantitative reverse transcription real-time polymerase chain reaction was used to determine the quantitative replication of viral RNA copies of neuraminidase gene of both AIV strains. A replication competence was observed according to which viral strain firstly inoculated. Whereas, H5N1 RNA titers was reduced by ≥4log10 when H5N8 firstly inoculated. Vice versa H5N8 RNA titers were reduced by ≥4log10 when H5N1 firstly inoculated. The interference impact of H5N1 was more powerful than that of H5N8 when simultaneous inoculation of both viruses was applied. In conclusion, quantitative evaluation of mixed inoculation of AIV strains exhibited replication competence of the first virus inoculation on the account of the succeeding one regardless of viral strain. However, H5N8 showed lower replication intensity according to the neuraminidase gene evaluation when both H5N1 and H5N8 were simultaneously inoculated in SPF ECE. Keywords: avian influenza virus, H5N1, H5N8, quantitative real-time polymerase chain reaction, viral interference.
Introduction
The poultry business sectors contribute significantly to the national market value by attracting private investment and creating jobs. Poultry meat remains an affordable source of animal nutrition for people of middle or low income (Abdelwhab & Hafez, 2011). Over several years, severe endemic respiratory virus infection resulted in catastrophic mortalities in the farm and backyard sectors. In majority of the afflicted poultry sectors, avian influenza (AI), Newcastle disease (ND), and infectious bronchitis (IB), either single or co-infection have been found; also, bacterial infections can occasionally exacerbate the situation (Hassan et al., 2016; Radwan et al., 2013; Samy & Naguib, 2018). Avian Influenza virus (AIV) has continued to circulate in Egyptian poultry farms and backyard since the first identification of AI virus (AIV) in poultry in 2006, when outbreaks of highly pathogenic (HP) AIV H5N1 of clade 2.2.1 were appeared (Aly et al., 2008; Peyre et al., 2009). over the course of years, frequent mutations in the hemagglutinin gene (HA) were occurred resulting in amino acid substitutions, which were linked to clade shifting (2.2.1, 2.2.1.2) and vaccine escape (2.2.1.1) (Balish et al., 2010; Hassan et al., 2016). The new clade 2.2.1.2 appeared in 2014–2015 resulted in great number of human infection (Arafa et al., 2016, WHO, 2019). The AI situation in Egypt has been further complicated by the recent arrival of antigenically different strain, HPAIV H5N8 of clade 2.3.4.4b, originated in China and spread across Central Asia into Europe and Africa reaching Egypt in 2016 (Selim et al., 2017). As well as, recent studies suggested that multiple introductions of different reassortants HPAI H5N8 viruses of clade 2.3.4.4b occurred in Egypt causing cases in wild birds (Yehia et al., 2018). These two subtypes of AIV appeared to be co-circulating in Egyptian poultry farms at the moment which both have been shown zoonotic potential (Who, 2019). In addition to the aforementioned a third co-circulated virus in the Egyptian poultry farms LPAI H9N2 has proven zoonotic potential (EFSA, 2018). During 2017 and 2018, a surveillance study found that H9N2 co-circulated with HPAIV H5N8 in 56.8% of flocks with 10 to 12 % sustained infection with HPAI H5N1. Reasons for the relative increase of HPAIV H5N8 infections versus H5N1 remained unclear, but lack of suitable vaccines against clade 2.3.4.4b cannot be excluded (Hassan et al., 2021). Evidence for high incidence of mixed infections of different viral diseases as AIV and Newcastle dis virus was provided by several studies (Amer et al., 2018 & El-lakany et al., 2018).The likelihood of viral competence between AIV and Newcastle disease virus (NDV) co-infection was discussed by a previous study (Soliman et al., 2019).The study has provided an evidence of replication competence between AIV H5N1 and H5N8 that revealed higher replication competence of H5N8 against H5N1 as well as both AIV strains showed higher replication competence against NDV that was based on quantitative evaluation of hemagglutinin (HA) gene in-vitro infection in SPF-ECE (Soliman et al., 2019). According to previous studies there are lack of information about the effect of neuraminidase NA gene in AIV on replication competence in case of co-infection of different AIV strains Therefore, the present study aims to figure out the effect of viral competence between H5N1 and H5N8 based on quantitative evaluation of neuraminidase NA gene replication. This study aimed to estimate quantitatively the viral interference of mixed infection between both circulated AIVs H5N1 and H5N8 in-vitro using qrRT-PCR. Materials and Methods Virus strain SPF-ECE inoculation
Experimental designs for interference study between H5N1 and H5N8
Table 1: Experimental design
a: Each group have 15 ECE, all groups were repeated in triplets. b: H5N1 and H5N8 the inoculated virus concentration was 103 EID50 c: Phosphate buffer saline PBS Quantitative Real Time PCR (qRT-PCR) for Neuraminidase gene N1or N8 Viral RNA was extracted from the harvested allantoic fluids by using QIAamp viral RNA mini kit (Qiagen, Germany) in accordance with manufacturer’s instructions. One-Step RT-PCR kit (Thermo scientific, USA) and specific oligonucleotide primers with taqman probes (Metabion, Germany) were used for detection and quantification of AIV-N1 (Li et al., 2013) and AIV-N8 (Hoffman et al.,2016). To determine AIV-H5N1 and AIV-H5N8 titers, a standard curve for each virus was generated using titrated viruses in SPF-ECE. The qRT-PCR reaction volume was 25 µl containing 4.5 µl of extracted RNA, 12.5 µl 2 × One-step RT-PCR ready mix, 1 µl RT enzyme, 0.5 µl of 50 pmol of both forward and reverse primers, 0.125μl of specific probe of 30 pmol conc. And 5.875 nuclease free water. The thermal profile included a reverse transcription step at 50 °C for 30 min followed denaturation step at 95 °C for 15 min. The PCR cycling was performed in 40 cycles of denaturation at 95 °C for 15s, annealing (55 °C both viruses) for 30s and extension at 72 °C for 10s, QRT-PCR was performed using Step One Plus Real-Time PCR machine (Applied Biosystem Thermo scientific, USA).Samples with a Cq value ≤39 were considered positive. Statistical analysis Results
Figure-1: The replication titers of sequential inoculation H5N1 then H5N8. Black and grey columns represent the positive control group (single H5N8 &H5N1 inoculation respectively). Two columns represent N1 and N8 titers of sequential inoculation (H5N1 then H5N8). Bars over the columns represent the error bars of standard deviation to the mean titers of triplicate values. p<0.05. The second group G2 experiment represented the sequential coinfection of first H5N8 then subsequent of H5N1. The AIV (H5N1) RNA titers showed reduction ≥4log10 compared with the control group (figure 2).
Figure-2: The replication titers of sequential inoculation H5N8 then H5N1 . Black and grey columns represent the positive control group. Two columns represent N8 and N1 titers. Bars over the columns represent the error bars of standard deviation to the mean titers. p<0.05 In the third group G3 ,AIV (H5N8) titers showed reduction when ECEs were simultaneously infected with H5N1 (figure-3). The titers of Neuraminidase (N8) of H5N8 RNA showed ≤4log10 reduction when co-inoculated with H5N1 compared to the RNA titer of N8 control. The titers of Neuraminidase (N1) of H5N1 RNA showed no reduction in simultaneous co-infection in ECE, as it showed equal titer 7 to 8 log10 with the control group.
Figure-3: The replication titers of simultaneous inoculations of H5N1 and H5N8. Black and grey columns represent the positive control group. Two columns represent N1 and N8 titers. Bars over the columns represent the error bars of standard deviation to the mean titers of triplicate values. p<0.05
Discussion Results showed superior competence of first inoculated viral strain. Reduction of AIV-N8 (≥4 log10) compared with the control after first inoculation of AIV-N1. However, the reduction of AIV-N1 after first inoculation of N8 was ≥4 log10 compared with control. Interestingly, the effect of N1 is more powerful of N8 in simultaneous infection which showed ≤4 log10 reduction of N8. The obtained results provide that the effect of viral competence was depending on the high replicative first infective strain regardless the type of N gene. The simultaneous infection of both N1 and N8 strains showed greater replication H5N1 than AIV-N8 which was contradictory with pattern of HA gene expression which showed greater expression of H5N8 than H5N1. In accordance to a related study (Ge et al., 2012), the primary infection by NDV virus succeeded to inhibit to a lesser extent (<1-2 log10) the later AIV replication. However, our result was corroborated by the previous research that supported the concept that pre-infection with a H5N1 can minimize virus replication of a later H5N8 and vice versa. A study showed that Quantitative results of AIV and IBV co-infection showed that interferences between the two viruses yielded decreased viral growth. It appears that either AIV or IBV has a negative impact on the other virus growth when they are inoculated simultaneously or sequentially (Aouini et al., 2018). A query was about the virulence of the viral strains that was important factor that affects interference in the current study. Previous studies Costa-Hurtado et al., 2014 & Dortmans et al., 2010 reported the direct correlation between NDV strain virulence and the degree of replication. In contrast the virulence was not shown in the study between H5N1 and H5N8, despite of the simultaneous infection showed increased replication of H5N1 over H5N8. A preceding study (Liu et al., 2003) also reported AIV- H9 interference due to NDV replication in ECEs. Another study (Costa-Hurtado et al., 2015) was performed in SPF chickens indicated that the previous infection of NDV can decrease the replication of second infection virus HPAIV of H5N2 subtype. These previous studies explained the pattern of replication competence was based on the basic classical theory of receptor competition that depend on the first virus grabbed most of receptors at the expense of second virus replication. Conclusions Recommendation | |||||||||||||||||||||||||||||||||||||||||||||||||
References | |||||||||||||||||||||||||||||||||||||||||||||||||
Abdelwhab, E. M., & Hafez, H. M. (2011). An overview of the epidemic of highly pathogenic H5N1 avian influenza virus in Egypt: Epidemiology and control challenges. Epidemiology & Infection, 139(5), 647–657.
Aly, M. M., Arafa, A., & Hassan, M. K. (2008). Epidemiological findings of outbreaks of disease caused by highly pathogenic H5N1 avian influenza virus in poultry in Egypt during 2006. Avian Diseases, 52(2),269–277. Amer, M.M., Maatouq, A.M., Abdel-Alim, G.A.,Awaad, M.H.H. and Kutkat, M.A. (2018) Isolation and Identification of H9N2 avian influenza and Newcastle disease viruses co-infections in chicken Egypt. Journal. Veterinary. Science, 49(2): 135-146. Aouini, R., Laamiri, N., &Ghram, A. (2018). Viral interference between low pathogenic avian influenza H9N2 and avian infectious bronchitis viruses in vitro and in ovo. Journal of virological methods, 259, 92–99.
Arafa, A., El‐Masry, I., Kholosy, S., Hassan, M. K., Dauphin, G., Lubroth,J., &Makonnen, Y. J. (2016). Phylodynamics of avian influenza clade2.2.1 H5N1 viruses in Egypt. Virology Journal, 13, 49–49. https://doi.org/10.1186/s12985‐016‐0477‐7
Balish, A. L., Davis, C. T., Saad, M. D., El‐Sayed, N., Esmat, H., Tjaden, J.A., Klimov, A. I. (2010). Antigenic and genetic diversity of highly pathogenic avian influenza A (H5N1) viruses isolated in Egypt. Avian Diseases, 54, 6.
Costa-Hurtado, M., Afonso, C.L., Miller, P.J., Shepherd, E.,Cha, R.M., Smith, D., Spackman, E., Kapczynski, D.R.,Suarez, D.L., Swayne, D.E. and Pantin-Jackwood, M.J. (2015)Previous infection with virulent strains of Newcastle disease virus reduces highly pathogenic avian influenza virus replication, disease, and mortality in chickens. Veterinary. Research, 46(1): 97.
Costa-Hurtado, M., Afonso, C.L., Miller, P.J., Spackman, E.,Kapczynski, D.R., Swayne, D.E., Shepherd, E., Smith, D.,Zsak, A. and Pantin-Jackwood, M. (2014) Virus interference between H7N2 low pathogenic avian influenza virus and lentogenic Newcastle disease virus in experimental co-infections in chickens and Turkeys. Veterinary. Research, 45(1): 1.
Dortmans, J.C.F., Rottier, P.J.M., Koch, G. and Peeters, B.P.H. (2010) The viral replication complex is associated with the virulence of Newcastle disease virus. Journal. Virology, 84(19) : 10113-10120.26.
Ellakany, H. F., Gado, A. R., Elbestawy, A. R., Abd El-Hamid, H. S., Hafez, H. M., Abd El-Hack, M. E., Swelum, A. A., Al-Owaimer, A., & Saadeldin, I. M. (2018). Interaction between avian influenza subtype H9N2 and Newcastle disease virus vaccine strain (LaSota) in chickens. BMC veterinary research, 14(1), 358.
European Food Safety Authority, European Centre for Disease Prevention and Control, European Union Reference Laboratory for Avian Influenza, Adlhoch, C., Brouwer, A., Kuiken, T., Mulatti, P., Smietanka, K., Staubach, C., Muñoz Guajardo, I., Verdonck, F., Amato, L., & Baldinelli, F. (2018). Avian influenza overview February - May 2018. EFSA journal. European Food Safety Authority, 16(6), e05358. https://doi.org/10.2903/j.efsa.2018.
Ge, S., Zheng, D., Zhao, Y., Liu, H., Liu, W., Sun, Q., Li, J.,Yu, S., Zuo, Y., Han, X., Li, L., Lv, Y., Wang, Y., Liu, X.and Wang, Z. (2012) Evaluating viral interference between Influenza virus and Newcastle disease virus using real-time reverse transcription polymerase chain reaction in chicken eggs. Virology. Journal, 9(128) : 1-8
Hassan, K.E., Shany, S.A., Ali, A., Dahshan, A.H.,El-Sawah, A.A. and El-Kady, M.F. (2016) Prevalence of avian respiratory viruses in broiler flocks in Egypt. Poultry Science, 95(6): 1271-1280. Hassan KE, El,Kady M, EL,Sawah AAA, et al. (2021) Respiratory disease due to mixed viral infections in poultry flocks in Egypt between 2017 and 2018: Upsurge of highly pathogenic avian influenza virus subtype H5N8 since 2018. Transboundary Emergency Diseases. 2021;68:21–36
Hoffmann, B.; Hoffmann, D.; Henritzi, D.; Beer, M. and Harder, T.C. (2016) .Riems influenza a typing array (RITA): An RT-qPCR-based low density array for subtyping avian and mammalian influenza a viruses. Scientific Reports, 6:27211, 1-9.
Li, L.H.; Yu, Z.; Chen, W.S.; Liu, S.L.; Lu, Y.; Zhang, Y.J.; Chen, E.F. and Lin, J.F. (2013): Evidence for H5 avian influenza infection in Zhejiang province, China, 2010-2012: a cross-sectional study. J Thorac Dis. 2013 Dec; 5(6):790-6.
Liu, W., Ding, W., Kong, J., Wang, H., Hu, S., He, H., Zhang, R. and Liu, X. (2003) The interference in the virus propagation in chicken embryo and in the HI test between the Newcastle disease virus and the H9 subtype influenza virus. Chin. Poultry Science 7(3) :1-6. OIE. World Organization for Animal Health. (2014). Chapter 2. 3. 4 (Avian influenza) in Manual of Diagnostic Tests and Vaccines for Terrestrial Animals. www.oie.int
Pantin-Jackwood, M.J., Costa-Hurtado, M., Miller, P.J., Afonso, C.L., Spackman, E., Kapczynski, D.R., Shepherd, E., Smith, D. and Swayne, D.E. (2015)Experimental co-infections of domestic ducks with a virulent Newcastle disease virus and low or highly pathogenic avian influenza viruses. Veterinary. Microbiology, 177(1-2): 7-17.
Peyre, M., Samaha, H., Makonnen, Y. J., Saad, A., Abd‐Elnabi, A., Galal,S., Domenech, J. (2009). Avian influenza vaccination in Egypt: limitations of the current strategy. Molecular Genetics and Medicine, 3,198–204. Radwan, M. M., Darwish, S. F., El-Sabagh, I. M., El-Sanousi, A. A., & Shalaby, M. A. (2013). Isolation and molecular characterization of Newcastle disease virus genotypes II and VIId in Egypt between 2011 and 2012. Virus genes, 47(2), 311–316. Reed, L.J. and Muench, H. (1938) A simple method of estimating fifty percent endpoints. Am. J. Epidemiol., 27(3): 493-497. Roussan, D.A., Haddad, R. and Khawaldeh, G. (2008) Molecular survey of avian respiratory pathogens in commercial broiler chicken flocks with respiratory diseases in Jordan. Poultry.Science, 87(3): 444-448. Samy, A., &Naguib, M. M. (2018). Avian respiratory coinfection and impact on avian influenza pathogenicity in domestic poultry: Field and experimental findings. Veterinary Sciences, 5(1), 23.
Selim, A. A., Erfan, A. M., Hagag, N., Zanaty, A., Samir, A. H., Samy, M., Abdelhalim, A., Arafa, A. A., Soliman, M. A., Shaheen, M., Ibraheem, E. M., Mahrous, I., Hassan, M. K., & Naguib, M. M. (2017). Highly Pathogenic Avian Influenza Virus (H5N8) Clade 2.3.4.4 Infection in Migratory Birds, Egypt. Emerging infectious diseases, 23(6), 1048–1051.
Soliman MA, Nour AA, Erfan AM (2019) Quantitative evaluation of viral interference among Egyptian isolates of highly pathogenic avian influenza viruses (H5N1 and H5N8) with the lentogenic and velogenic Newcastle disease virus genotype VII in specific pathogen-free embryonated chicken eggs model, Veterinary World, 12(11): 1833-1839.
WHO. (2019). Cumulative number of confirmed human cases for Avian Influenza A(H5N1) reported to WHO 2003–2019. Source: WHO/GIP,data in HQ as of 12 February 2019; https://www.who.int/influenza/human_animal_interface/2019_02_12_tableH5N1.pdf Yehia N., Naguib M. M., Li R., Hagag N., El-Husseiny M., Mosaad Z., Nour A., Rabea N., Hasan W. M., Hassan M. K., Harder T., Arafa A. A.. 2018. Multiple introductions of reassorted highly pathogenic avian influenza viruses (H5N8) clade 2.3.4.4b causing outbreaks in wild birds and poultry in Egypt. Infect. Genet. Evol.58:56–65. | |||||||||||||||||||||||||||||||||||||||||||||||||
Statistics Article View: 80 PDF Download: 62 |
|||||||||||||||||||||||||||||||||||||||||||||||||