Phenotypic and Genotypic Characterization of E. Coli Isolated From Fish and Human | ||||
| Benha Veterinary Medical Journal | ||||
| Article 5, Volume 34, Issue 2, June 2018, Page 41-50 PDF (445.8 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/bvmj.2018.29411 | ||||
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| Authors | ||||
| Ashraf A. AbdEl-Tawab1; Fatma I. El-Hofy1; Adel M. El-Gamal2; Heba O. Ibrahim3 | ||||
| 1Bacteriology, Immunology and Mycology Department Faculty of Veterinary Medicine, Benha University, Egypt | ||||
| 2BacteriologyDept, Animal Health Research Institute, Kafr El-Sheikh branch. Egypt | ||||
| 3Veterinary Medicine Directorate, Kafr El-Sheikh branch. Egypt | ||||
| Abstract | ||||
| The present work aimed to isolate and characterize E. coli bacteria that transmitted from fish to fish handlers in markets and farm workers, for this 200 apparently healthy Oreochromis niloticus were collected randomly from different markets and farms and 50 human skin swabs from sellers at markets and workers at farms were obtained at Kafr El-sheikh governorate, Egypt. These samples were cultured and biochemical characters was studied. Serological identification, antibiogram activity and molecular characterization of some virulence factors were detected. Results, the prevalence of Escherichia coli was 17.5% in Tilapia while its prevalence in human was 24%. Escherichia coli serotypes from fish were O153 :H2, O1 :H7, O125 :H21 and O78. Only one from four isolates of Escherichia coli was positive to eaeA gene and the four isolates were negative to hly gene. | ||||
| Keywords | ||||
| E. coli; biochemical; in vitro sensitivity; Virulence genes; Transmission; fish; human | ||||
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