IL 13rs20541 Single Nucleotide Polymorphism and Serum IL -13 Level in Children with Bronchial Asthma | ||||
| The Egyptian Journal of Pediatric Allergy and Immunology | ||||
| Volume 21, Issue 1, April 2023, Page 27-33 PDF (299.78 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejpa.2023.170190.1045 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Mona Adel Khattab 1; Mahitab Morsy Hussein  2; Walaa Shawky Khater3
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| 1Medical Microbiology & Immunology department, Faculty of Medicine Ain Shams University | ||||
| 2Department of pediatrics, faculty of medicine, Ain shams university, Cairo, Egypt | ||||
| 3Medical microbiology and immunology department, faculty of medicine, Ain shams university | ||||
| Abstract | ||||
| Background: Bronchial asthma is a common respiratory illness affecting adult and pediatric populations. Interleukin (IL)-13 is a central key mediator of allergic inflammation, and it plays a pivotal role in the pathogenesis of asthma. Several studies revealed that genetic variants of IL-13 promoter rs1800925 allele play a role in asthma severity. We sought to investigate the role of IL-13 rs20541 single nucleotide polymorphism (SNP) and serum IL -13 level in relation to asthma severity in a group of asthmatic children. Methods: This controlled cross-sectional study included 80 participants: 40 apparently healthy controls and 40 asthmatic children, subdivided into three groups: mild (n=12), moderate (n=18 cases), and severe (n=10), recruited from Pulmonology Unit, Children’s Hospital, Ain shams university. Characterization of IL-13 rs20541 SNP was achieved by TaqMan real-time PCR. Serum IL-13 concentration was measured using a commercial sandwich ELISA assay. Results: The dominant genotype as regards IL-13 rs20541 SNP among patients and controls was GA (60% and 92.5%, respectively). G and A allele frequencies between patients and controls differed significantly (p =0.002) as the dominant allele in the control group was G allele (70.0%), versus the A allele in the patients’ group (53.8%). Patients had statistically significantly higher levels of IL-13 compared to controls (median= 45 pg/ml versus 4 pg/ml; p<0.001) Serum IL-13 correlated positively with IgE levels and was able to discriminate between patients with severe asthma and those with mild to moderate asthma at cut off value of >83 pg/ml (sensitivity 90%, specificity 90%, positive predictive value 96.4% and negative predictive value 75%). Conclusion: IL-13 rs20541 SNP polymorphism is associated with asthma in Egyptian children, while serum IL-13 is linked to the severity of asthma | ||||
| Keywords | ||||
| Alleles; allergy; Genotypes; SNP; cytokine | ||||
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