Biomimetic Human Enamel Remineralization Using Calcium Extract from Nano Fish Bone and Eggshell: An In vitro Study | ||||
| Egyptian Dental Journal | ||||
| Article 7, Volume 69, Issue 2 - Serial Number 5, April 2023, Page 1603-1611 PDF (889.54 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/edj.2023.175109.2349 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Mona Essa   1; Amr Mostafa El-Bolok 2; Hoda Hassan Hammad 3
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| 1Associate Professor, Opertive Dentistry Department, Faculty of dentistry,Beni-Suef university | ||||
| 2Professor, Department of Oral Pathology, Faculty of Dentistry, Minia University, Minia, Egypt | ||||
| 3Assistant Professor, Dental Biomaterial, Faculty of Dentistry, Menoufia University, Egypt | ||||
| Abstract | ||||
| The study aimed to investigate remineralizing potential of two organic biomimetic waste nanomaterials [fish bone (FB) and chicken eggshell (ES)] on demineralized enamel using Energy Dispersive X-ray spectroscopy (EDX) for quantitative calcium/ phosphate assessment. A total of 34 freshly extracted human 3rd molars were collected to be used. Thirty teeth were divided into three equal groups (n=10). Group1: Teeth represented the control group as a reference for the assessed normal quantitative content of calcium and phosphorus. Group2: Teeth were immersed in a freshly prepared demineralizing solution for 96 hours, then investigated by EDX. Afterword, those group 2 teeth were again soaked in a freshly elaborated remineralizing fish bone nanoparticle solution for 189 hours, and then reevaluated by EDX. Group3: Teeth were demineralized with the same regime as group 2 teeth, then remineralized in a freshly prepared ES nanoparticle solution for seven days to be again reassessed by EDX. In addition, the remaining four human teeth were assessed by a scanning electron microscope (SEM); where 1 sound enamel surface was scanned, a second tooth was SEM investigated following demineralization regime, a third one was SEM assessed after remineralization with FB nanoparticle solution and fourth one scanned after ES nanoparticle remineralization. Paired sample t-test and independent t test statistical analysis were used. Freshly prepared nano FB and ES solutions seem promising for remineralization of incipient enamel lesions. | ||||
| Keywords | ||||
| Biomimetic; Demineralization; nanoparticles; Eggshell; Fish Bone | ||||
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