Effect of Protein Repellent Addition to Self-Healing Experimental Resin Composite on Microcosm Biofilm and Cytotoxicity | ||||
| Al-Azhar Dental Journal for Girls | ||||
| Article 6, Volume 10, Issue 2, April 2023, Page 325-334 PDF (884.56 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/adjg.2023.103800.1448 | ||||
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| Authors | ||||
Passant S. Abdel Hameed  1; Inas T. Motawea2; Hisham Essawy3; Mohamed Hosny4
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| 1Associate Lecturer of Dental Biomaterials, Al Zagazig University, Egypt | ||||
| 2Professor of Dental Biomaterials, Faculty of Dental Medicine for Girls, Al-Azhar University, Cairo, Egypt | ||||
| 3Professor in department of Polymer and Pigment chemistry, National Research Center, Cairo, Egypt | ||||
| 4Professor of Microbiology, Regional Center for Mycology and Biotechnology, Al-Azhar University, Cairo, Egypt | ||||
| Abstract | ||||
| Purpose: To investigate the effect of addition of MPC protein repellent agent to selfhealing experimental resin composite on the self-healing efficacy, microcosm biofilm and the cytoxicity. Material and Methods: Polyurea formaldehyde microcapsules were synthesized by Insitu Polymerization Technique. MPC and the microcapsules are added to the prepared experimental resin composite with different MPC concentrations. Protein repellence essay was examined by the Micro bicinchoninic Acid method. Biofilm models formed of streptococcus mutans growth was prepared and visualized by confocal laser electron microscope to investigate the live \ dead assay over the studied specimens. The cytotoxicity of the specimens was examined via SRB assay. Fracture toughness was examined using universal testing machine till fracture. Self-healing efficacy is evaluated. Results: In protein deposition, it was found that the 0%MPC has the highest value while the least value was found in the 10% group. In live/ dead bacterial assay, it was found that the 0%MPC has the highest living bacterial value and the lowest dead bacterial value while the 7.5% group has the lowest living bacterial value and the highest dead bacterial value. In the mechanical evaluation, the virgin fracture toughness, the highest facture toughness was found in the control specimens while the least fracture toughness was found in 10% group, the selfhealing efficacy results showed that the highest self-healing value was found in the 0%MPC while the least self-healing efficacy value was found in the control group. Conclusion: Innovative self-healing composite with protein repellence give rise for a great variety of dental biomaterials with more promising results and long clinical serviceability | ||||
| Keywords | ||||
| Self-healing; Protein repellent; Biofilm models; confocal electron scanning; cytotoxicity | ||||
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