Automated ID & AST using VITEK 2 and Prevalence of K. pneumoniae Isolated from diarrheic chicken in Sharqiyah Governorate
Shaikhon, F., Khafagy, A., Eidaros, N., Khalil, W., Yousseff, F., Ahmed, M. (2023). Automated ID & AST using VITEK 2 and Prevalence of K. pneumoniae Isolated from diarrheic chicken in Sharqiyah Governorate. EKB Journal Management System, (), -. doi: 10.21608/scvmj.2023.193755.1119
Fatma Elzahraa S Shaikhon; Ahmed Khafagy; Nada Eidaros; Waleed Khalil; Fatma Yousseff; Mona A. Ahmed. "Automated ID & AST using VITEK 2 and Prevalence of K. pneumoniae Isolated from diarrheic chicken in Sharqiyah Governorate". EKB Journal Management System, , , 2023, -. doi: 10.21608/scvmj.2023.193755.1119
Shaikhon, F., Khafagy, A., Eidaros, N., Khalil, W., Yousseff, F., Ahmed, M. (2023). 'Automated ID & AST using VITEK 2 and Prevalence of K. pneumoniae Isolated from diarrheic chicken in Sharqiyah Governorate', EKB Journal Management System, (), pp. -. doi: 10.21608/scvmj.2023.193755.1119
Shaikhon, F., Khafagy, A., Eidaros, N., Khalil, W., Yousseff, F., Ahmed, M. Automated ID & AST using VITEK 2 and Prevalence of K. pneumoniae Isolated from diarrheic chicken in Sharqiyah Governorate. EKB Journal Management System, 2023; (): -. doi: 10.21608/scvmj.2023.193755.1119

Automated ID & AST using VITEK 2 and Prevalence of K. pneumoniae Isolated from diarrheic chicken in Sharqiyah Governorate

Suez Canal Veterinary Medical Journal. SCVMJ
Articles in Press, Accepted Manuscript, Available Online from 16 April 2023  XML
Document Type: Original Article
DOI: 10.21608/scvmj.2023.193755.1119
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Authors
Fatma Elzahraa S Shaikhon1; Ahmed Khafagy email 2; Nada Eidaros3; Waleed Khalilorcid 4; Fatma Yousseff5; Mona A. Ahmed6
1Free Veterinarian
2Professor of Microbiology, Faculty of Veterinary Medicine, Suez Canal University
3Dep. of Bacteriology, Faculty of Veterinary Medicine, Suez Canal University
4Professor of Pharmacology Faculty of Veterinary Medicine, Suez Canal University
5Pathology Department, Animal Health Research Institute, Ismailia branch
6Bacteriology Department, Animal Health Research Institute, Ismailia branch
Abstract
The present study was conducted for the isolation of bacterial pathogens that affected the gastrointestinal tract of chickens and economically affect their production. In this study 100 cloacal samples were collected from diarrheic chickens from 4 different private farms in Bilbies Sharqiyah Governorate and examined bacteriologically for its cause. The results revealed that there were 5 isolates: 39% E. coli, 22% C. perfringens,14% K. pneumoniae and 14% P. mirabilis, 11% P.vulgaris along with no detection of Salmonellae (0%). All 14 K. pneumoniae isolates previously identified using the traditional methods were identified rapidly and automatically by VITEK 2 system (bioMerieux, Craponne, France) using AST and ID-GNB cards, Vitek 2 software, Advanced Expert System [AES] software, and all 14 isolates were sensitive for 24 different antimicrobial agents: Cefazolin, Cefepime, Ciprofloxacin, Gentamycin, Tobramycin, Cefuroxime, Piperacillin, Amikacin, Meropenen, Cefotaxime, Trimethoprim sulfa, Ceftazidime, Levofloxacin, Chloramphenicol, Imipenem, Aztreonam, Cefoxitin, Amoxicillin/CA, Ticarcillin/CA, Minocycline, Ertapenem, Tigecycline, Moxifloxacin, Ampicillin/sulbactam.
Keywords
E. coli; C. perfringens; K. pneumoniae; P. mirabilis; P.vulgaris & VITEK 2 automated system
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