Virucidal effect of Moringa oleifera against SARS-CoV-2 and Influenza A/H1N1
Allam, O., Kutkat, O., Gaballah, M., El-Halawany, A., Mostafa, A., Shouman, S., Ali, M., El Farouk, O. (2023). Virucidal effect of Moringa oleifera against SARS-CoV-2 and Influenza A/H1N1. EKB Journal Management System, 19(1), 69-78. doi: 10.21608/AJBS.2023.197628.1053
Omnia Gamal Allam; Omnia Kutkat; Mohamed Gaballah; Ali M. El-Halawany; Ahmed Mostafa; Sahar Shouman; Mohamed A. Ali; Omar El Farouk. "Virucidal effect of Moringa oleifera against SARS-CoV-2 and Influenza A/H1N1". EKB Journal Management System, 19, 1, 2023, 69-78. doi: 10.21608/AJBS.2023.197628.1053
Allam, O., Kutkat, O., Gaballah, M., El-Halawany, A., Mostafa, A., Shouman, S., Ali, M., El Farouk, O. (2023). 'Virucidal effect of Moringa oleifera against SARS-CoV-2 and Influenza A/H1N1', EKB Journal Management System, 19(1), pp. 69-78. doi: 10.21608/AJBS.2023.197628.1053
Allam, O., Kutkat, O., Gaballah, M., El-Halawany, A., Mostafa, A., Shouman, S., Ali, M., El Farouk, O. Virucidal effect of Moringa oleifera against SARS-CoV-2 and Influenza A/H1N1. EKB Journal Management System, 2023; 19(1): 69-78. doi: 10.21608/AJBS.2023.197628.1053

Virucidal effect of Moringa oleifera against SARS-CoV-2 and Influenza A/H1N1

African Journal of Biological Sciences
Volume 19, Issue 1, June 2023, Page 69-78  XML PDF (281.63 K)
Document Type: Original Article
DOI: 10.21608/AJBS.2023.197628.1053
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Authors
Omnia Gamal Allam email 1; Omnia Kutkat2; Mohamed Gaballah2; Ali M. El-Halawany3; Ahmed Mostafa2; Sahar Shouman4; Mohamed A. Ali2; Omar El Farouk4
1Microbiology, faculty of science, Ain Shams university, Cairo, Egypt
2Center of Scientific Excellence for Influenza Viruses, National Research Centre, Giza 12622, Egypt
3Department of Pharmacognosy, Faculty of Pharmacy, Cairo University,
4Department of Microbiology, Faculty of Science, Ain Shams University, Cairo 11566, Egypt.
Abstract
  In the last two decades, global human health problems and economic disasters have been brought on by many viruses, such as severe acute respiratory syndrome coronavirus 1 (SARS-CoV-1), severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), and middle east respiratory syndrome coronavirus (MERS-CoV), belonging to coronaviruses, and influenza viruses mainly H1N1. The lack of specific therapies and few available treatments for these viruses have made them more difficult to overcome. A significant barrier to antiviral therapy is the emergence of drug-resistant respiratory viruses; as a result of naturally occurring mutations, in addition to the misuse of already approved antiviral drugs. Therefore, this study will be on Moringa oleifera leaf extract as one of the most common medicinal plants. The ethanolic extract of the plant leaf powder was prepared and fractionated, later the crude and the fractions were tested for antiviral activity against H1N1 and SARS-CoV-2 as the causative agent for pandemic 2009 and 2019, respectively, after safety confirmation on Madin-Darby canine kidney (MDCK) and African green monkey kidney derived cells (Vero-E6) cell lines. The plaque reduction assay was performed for the crude ethanolic extract to determine the action of the antiviral medication with a low IC50. The results showed high safety on the two cell types and the activity against both viruses with high percentage of direct inhibition (virucidal), indicating that Moringa oleifera can represent a promising antiviral agent against them.
 
Keywords
SARS-CoV-2; H1N1; Moringa oleifera; virucidal effect; Vero-E6 cells
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