Acaricidal Effects of Selamectin and Ficus sycomorus extracts on Sarcoptes scabiei Mites Infection in Rabbit | ||||
| Journal of Current Veterinary Research | ||||
| Article 15, Volume 5, Issue 1, April 2023, Page 206-222 PDF (1.09 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jcvr.2023.296057 | ||||
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| Authors | ||||
| Ahmed Dawod* ; Said I. Fathalla ; Ahmed Elkhatam; Nagwa I. Sheraiba; Mohamed A. Hammad; Hesham R. El-Seedi ; Awad A. Shehata; Anis Anis; Marwa A.A. Fayed; Noha Osman | ||||
| Abstract | ||||
| This study aimed to evaluation of the in vitro and in vivo acaricidal activity of the Ficus sycomorus (F. sycomorus) against fur mites in rabbits. Methanol 70% was used to extract F. sycomorus extracts from either the leaf or stem bark. The in vitro acaricidal activity of F. sycomorus extracts was tested using 2 ml of each F. sycomorus extract at 0.125, 0.25, 0.5, and 1 mg/ml concentrations in each experimental Petri dish containing ten Sarcoptesscabiei (S. scabiei) mites/dish. Negative control mites were given distilled water, whereas positive control mites were given selamectin. The stereomicroscopic examination was then performed 30 minutes after incubation and was repeated hourly until 7 hours after incubation. The mortality rate, median lethal time (LT50), and lethal concentration (LC50 and LC99) were calculated. Also, for in vivo acaricidal activity of the same extracts were done using fifty intact New Zealand white male rabbits allocated to five experimental groups (n = 10). The first experimental group kept without infection as the negative control group. Whereas, the rabbits in other groups were inoculated with 50S. scabiei mites previously isolated on the skin of the tip of the left ear auricle. After the infection confirmation, the second group was treated topically with distilled water and kept as the positive control group. Also, the third group was treated with selamectin at a dose rate of 12 mg/kg BW on days 0, and 14th during treatment and served as a drug-treated group. While the fourth and the fifth groups were subjected to 9 drops (0.6 ml) of 100 mg/ml daily of either F. sycomorus leaf or stem bark extracts topically on the fur mite lesions every other day up to day 21. The results of the study revealed a promising in vitroacaricidal activity of F. sycomorus leaf and bark extracts on S. scabiei mites and this effect was time and dose-dependent. LC50 and LC99 of S. scabiei at 4 hours after F. sycomorus leaf extract treatment were 0.149 and 1.373 g/ml, while for the bark extract were 0.124 and 1.074. This effect was confirmed in the in vivo trials as either leaf or stem bark extracts of F. sycomorus showed an effective acaricidal activity against S. scabiei mites. | ||||
| Keywords | ||||
| Acaricidal effects; Ficus sycomorus; Rabbits; Sarcoptesscabiei and Selamectin. | ||||
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