ANTIMICROBIAL AND ANTIOXIDANT POTENTIALS OF ENDOPHYTIC ACTINOMYCETES ISOLATED FROM LEAVES OF ASPHODELUS TENUIFOLIUS (CAV.) (MARSA MATROUH, EGYPT) | ||||
Egyptian Journal of Desert Research | ||||
Article 7, Volume 66, Issue 2, December 2016, Page 373-386 PDF (750.71 K) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/ejdr.2016.29639 | ||||
View on SCiNiTO | ||||
Authors | ||||
Dina M. Fahmy 1; Nagwa Sidkey2; Mai Elkawaga2; Doaa S. Zaid2 | ||||
1Natural Products Unit, Department of Medicinal and Aromatic Plants, Desert Research Center, El-Matareya, Cairo, Egypt | ||||
2Department of Botany and Microbiology, Faculty of Science, Al-Azhar University, Cairo, Egypt | ||||
Abstract | ||||
The study aimed to evaluate the antimicrobial and antioxidant abilities of four endophytic actinomycetes (strain Act1, Act2, Act3, and Act4) isolated from leaves of Asphodelus tenuifolius, as well as assessment of their phytochemical profile. Referring to morphological characters and 16S rDNA sequence analysis, actinomycete (strain Act2) was identified as Streptomyces fenghuangensis (GIMN4.003T) with 96% identical sequence and accession number (NR1175021). The results showed variant inhibitory activity against pathogenic bacteria and fungi. Act2endophytic actinomycete isolate showed broad spectrum activity against all pathogenic tested organisms, recording notable MIC value 10 µg/ml against Bacillus subtilus andBacillus cereus, while At3and At4showed strong impact on Candida albicans. All of the ethyl acatate extracts of actinomycete isolates revealed high antioxidant activity ranging from 45-82% using DPPH radical. Phytochemical screening of the isolates indicates the presence of phenolics, flavonoids, sterols, anthraquinones and naphthaquinones. | ||||
Keywords | ||||
Asphodelus tenuifolius; Antimicrobial; antioxidant; Streptomyces fenghuangensis; Phytochemical screening | ||||
Statistics Article View: 308 PDF Download: 360 |
||||