A Comprehensive Protocol for In Vitro Propagation and Acclimatization of Jacaranda mimosifolia Trees under the Egyptian Conditions
Sabah, S., Hosni, A., Abdul Raziq, A., Hewidy, M. (2022). A Comprehensive Protocol for In Vitro Propagation and Acclimatization of Jacaranda mimosifolia Trees under the Egyptian Conditions. EKB Journal Management System, 73(4), 79-92. doi: 10.21608/ejarc.2023.296441
Saleh S. Sabah; Abdelaziz M. Hosni; Ashraf B. Abdul Raziq; Mohammed Hewidy. "A Comprehensive Protocol for In Vitro Propagation and Acclimatization of Jacaranda mimosifolia Trees under the Egyptian Conditions". EKB Journal Management System, 73, 4, 2022, 79-92. doi: 10.21608/ejarc.2023.296441
Sabah, S., Hosni, A., Abdul Raziq, A., Hewidy, M. (2022). 'A Comprehensive Protocol for In Vitro Propagation and Acclimatization of Jacaranda mimosifolia Trees under the Egyptian Conditions', EKB Journal Management System, 73(4), pp. 79-92. doi: 10.21608/ejarc.2023.296441
Sabah, S., Hosni, A., Abdul Raziq, A., Hewidy, M. A Comprehensive Protocol for In Vitro Propagation and Acclimatization of Jacaranda mimosifolia Trees under the Egyptian Conditions. EKB Journal Management System, 2022; 73(4): 79-92. doi: 10.21608/ejarc.2023.296441

A Comprehensive Protocol for In Vitro Propagation and Acclimatization of Jacaranda mimosifolia Trees under the Egyptian Conditions

Egyptian Journal of Agricultural Sciences
Volume 73, Issue 4, October 2022, Page 79-92  XML PDF (938.68 K)
Document Type: Research articles
DOI: 10.21608/ejarc.2023.296441
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Authors
Saleh S. Sabah email 1; Abdelaziz M. Hosniorcid 2; Ashraf B. Abdul Raziq3; Mohammed Hewidy4
1Department of Desertification, Faculty of Agriculture, Al-Muthana University, Iraq
2Horticulture Department, Faculty of Agriculture, Ain Shams University, P.O. Box 68, Hadayek Shubra, Cairo 11241, Egypt
3Genetic Department, Faculty of Agriculture, Ain Shams University, 11241- Cairo, Egypt.
4Horticulture Department, Faculty of Agriculture, Ein Shams University, 11241-Cairo Egypt
Abstract
     This study was carried out during the autumn season of 2021-2022 to establish a detailed protocol for the micropropagation of Jacaranda mimosifolia trees. The best results in terms of eliminating contamination (0.0%) were obtained by soaking seeds in Clorox (NaOCl) solution for 9 or 12 min. The highest seeds survival rate (100%) was obtained when sterile seeds were planted in ¾ strength MS medium + 30 g/l sucrose + 6% agar, and pH 5.7. The results showed that ½ strength MS was a suitable medium for the next multiplication stage, because it later gave the highest number of shoots, and chlorophyll A content. On the other hand, the ¼ strength MS medium was suitable for the rooting stage because it resulted in the best root length and shoot length. In terms of shoot length and fresh weight were obtained with 2.0 mg/l 6-BAP + 0.0 mg/l NAA, while 4.0 mg/l 6-BAP + 0.0 mg/l NAA gave the highest number of shoots, and 1.0 mg/l 6-BAP + 0.0 mg/l NAA resulted in the best flavonoids content. The highest root number and dry weight were obtained with 2.0 g/l AC + 0.5 mg/l NAA, while the longest shoots were obtained with 0.0 g/l AC + 0.1 mg/l NAA. In the acclimatization stage, the best plant height was obtained with Peat moss + Perlite at a ratio of 2:1 (v/v). The survival percentage (100%) was equal in both of the tested acclimatization media, viz., Peat moss + perlite at 2:1 (v/v) and Peat moss + perlite + vermiculite at 2:1:1 (v/v/v). The economic feasibility and production costs were calculated after determining the appropriate media for the different micropropagation stages of J. mimosifolia.
Keywords
Jacaranda mimosifolia; micropropagation; in vitro propagation; acclimatization
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