Ameliorative effect of Silybum marianum extract "Milk thistle" against Lithium-induced cardiac toxicity in adult male albino rats.
Fekry, E., Awny, M., Refaat, G., Arafat, H. (2023). Ameliorative effect of Silybum marianum extract "Milk thistle" against Lithium-induced cardiac toxicity in adult male albino rats.. EKB Journal Management System, 31(2), 17-33. doi: 10.21608/mjfmct.2023.201132.1061
Ereny Fekry; Mona Awny; George Refaat; Horeya Arafat. "Ameliorative effect of Silybum marianum extract "Milk thistle" against Lithium-induced cardiac toxicity in adult male albino rats.". EKB Journal Management System, 31, 2, 2023, 17-33. doi: 10.21608/mjfmct.2023.201132.1061
Fekry, E., Awny, M., Refaat, G., Arafat, H. (2023). 'Ameliorative effect of Silybum marianum extract "Milk thistle" against Lithium-induced cardiac toxicity in adult male albino rats.', EKB Journal Management System, 31(2), pp. 17-33. doi: 10.21608/mjfmct.2023.201132.1061
Fekry, E., Awny, M., Refaat, G., Arafat, H. Ameliorative effect of Silybum marianum extract "Milk thistle" against Lithium-induced cardiac toxicity in adult male albino rats.. EKB Journal Management System, 2023; 31(2): 17-33. doi: 10.21608/mjfmct.2023.201132.1061

Ameliorative effect of Silybum marianum extract "Milk thistle" against Lithium-induced cardiac toxicity in adult male albino rats.

Mansoura Journal of Forensic Medicine and Clinical Toxicology
Volume 31, Issue 2, July 2023, Page 17-33  XML PDF (2.43 MB)
Document Type: Original Article
DOI: 10.21608/mjfmct.2023.201132.1061
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Authors
Ereny Fekryorcid 1; Mona Awny email orcid 2; George Refaatorcid 3; Horeya Arafatorcid 1
1Department of Histology and Cell Biology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt.
2Department of Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt.
3Departments of Internal Medicine & Cardiology, Suez Canal Authority, Ismailia, Egypt.
Abstract
Abstract:
Lithium carbonate is the first line of treatment for bipolar disorder. With its narrow therapeutic index and prolonged therapeutic doses, it causes multiple toxic effects. Cardiotoxicity is one of the serious toxicities induced by lithium treatment. Silybum marianum; an Asteraceae plant; with Silymarin as its major active constituent, has antioxidant, anti-inflammatory, and immunomodulatory properties. To assess the ameliorative effect of Silybum marianum extract “Milk thistle” on the histopathological changes of the cardiac muscle caused by lithium in adult male albino rats, using histological and immunohistochemical techniques. Twenty-Four adult male albino rats were equally randomized into 4 groups and received all treatments by oral gavage for 4 weeks. Group I (control) received distilled water. Group II received Silybum marianum extract (200 mg/kg/day). Group III received Lithium carbonate (50 mg/Kg). Group IV, Lithium carbonate concomitant with Silybum marianum extract was given. All animals were sacrificed after 4 weeks by decapitation. Prepared sections were subjected to hematoxylin and eosin stain, Mallory’s trichrome stain, iron hematoxylin, and KI-67 immunohistochemistry. lithium-induced widely disorganized and separated cardiac muscle fibers with pyknotic nuclei, without visible striations, increased collagen deposition and showed negative Ki-67 nuclear immune reaction. The use of Silybum marianum extract “Milk thistle” reversed cardiac muscle fibers alterations, decreased collagen amount, and restored Ki-67 nuclear immune reaction. Silybum marianum extract “Milk thistle” protects against lithium-induced cardiotoxicity in rats.
Keywords
Cardiotoxicity; Lithium carbonate; histological stains; cardiac muscle; Ki-67
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