Histological study on the possible protective role of L-carnitine against testicular ischemia-reperfusion injury induced in rabbits | ||||
| Egyptian Journal of Histology | ||||
| Articles in Press, Accepted Manuscript, Available Online from 30 April 2023 | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejh.2023.199635.1873 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Mohamed Abdel Rahman1; Hala Madkour2; Fatma El-saeed El-Demerdash3; Hala Mahfouz4; Abdelrahman Ahmed Aburahma5; Sarah Mosaad Amer  6
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| 1Department of Human Anatomy and Embryology, Faculty of Medicine, Sohag University, Sohag, Egypt | ||||
| 2Department of Pharmacology, Faculty of Medicine, Sohag University, Sohag, Egypt | ||||
| 3Department of Zoology and Entomology, Faculty of Science (Girl's),Al-Azhar University, Cairo, Egypt | ||||
| 4Department of medical biochemistry and molecular biology, Faculty of Medicine, Kafrelsheikh University, Kafrelsheikh, Egypt | ||||
| 5Department of Andrology, Faculty of Medicine, Cairo University, Manial, Cairo, Egypt | ||||
| 6Department of Medical Histology and Cell Biology, Faculty of Medicine, Cairo University, Manial, Cairo, Egypt | ||||
| Abstract | ||||
| Abstract: Background: The torsion of the testis is a surgical emergent situation which is common; however there are no effective measures to avoid damage of the testis after untwisting this torsion. L-carnitine (LC) is an essential micronutrient with strong anti-apoptotic, anti-inflammatory and antioxidant potencies that can reverse oxidative stress induced testicular damage. Aim of the work: To evaluate the potential protective role of LC against the testicular damage induced by ischemia reperfusion (IR) injury in rabbits. Materials and methods: 50 adult male rabbits weighing 1300-1600 g were divided into 5 groups (10 rabbits per group). Acute IR testicular injury was induced in rabbits by twisting the left spermatic cord 720º for 2 h. Group 1: Control group and divided into two subgroups (subgroup A negative control and subgroup B positive control); Group 2: Sham-operated; Group 3: Ischemia group; Group 4: IR group (testicular IR was induced without LC); Group 5: IR+LC testicular IR was induced and LC was given by intra-peritoneal injection at a dose of 500 mg/kg immediately after torsion. At the experimental end, tissue samples of left testes were collected from all rabbits of the five groups and evaluated biochemically and histologically. Results: Ischemia reperfusion resulted in disturbance of the oxidant/antioxidant status markers, loss of normal architecture of spermatogenic cells, apparent increase in the number of caspase-3 positive spermatogenic cells as well as abnormal fragmented chromatin material in addition to lack of halos around the sperm nuclei. However, the intra-peritoneal administration of LC immediately after torsion significantly improved these changes caused by IR on the testes. Conclusion: Administration of LC reduced the testicular damage induced by the ischemia-reperfusion. Key Words: L-carnitine, Testes, Ischemia-Reperfusion injury, Rabbits. | ||||
| Keywords | ||||
| L-carnitine; Testes; Ischemia-Reperfusion injury; Rabbits | ||||
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