Extracellular biosynthesis and antimicrobial activity of Bacillus subtilis ATCC 6633 zinc oxide nanoparticles | ||||
| Scientific Journal for Damietta Faculty of Science | ||||
| Volume 13, Issue 2, 2023, Page 39-47 PDF (693.22 K) | ||||
| Document Type: Original articles | ||||
| DOI: 10.21608/sjdfs.2023.173178.1064 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Amira T. Abd El-Nour; Mohamed I. Abou-Dobara; Ahmed K.A. El-Sayed; Mohamed M. El-Zahed  
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| Department of Botany and Microbiology, Faculty of Science, Damietta University, Egypt. | ||||
| Abstract | ||||
| Recently, zinc oxide nanoparticles (ZnO NPs) have been used in several biological and industrial applications due to their low toxicity, high efficiency, biocompatibility, and cost-effectiveness. The present study used Bacillus subtilis ATCC 6633 in the biosynthesis of the antimicrobial ZnO NPs. The synthesized ZnO NPs were characterized using UV-Visible spectroscopy, Fourier transform-infrared (FTIR) spectroscopy, X-ray diffraction (XRD) spectroscopy, Zeta potential analysis, and transmission electron microscopy (TEM). The TEM results confirmed the spherical shape of ZnO NPs with a size range of 13.5-30.4 nm. Zeta potential analysis showed the positive charge of ZnO NPs (+16.8 mV). The ZnO NPs showed good antimicrobial activity against the tested pathogenic bacterial strains; B. cereus (Gram-positive bacteria) in comparison to Escherichia coli (Gram-negative bacteria). They also revealed a high antifungal activity against the tested pathogenic fungal strain; Aspergillus niger. Here, this cost-effective and simple biological approach for ZnO NPs synthesis has emerged with a promising capacity in biomedicine, especially in the fields of antibacterial and antifungal fields. | ||||
| Keywords | ||||
| Zinc oxide; nanoparticles; biosynthesis; characterization; antimicrobial activity | ||||
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