Molecular characterization of extended spectrum β-lactamase resistance genes for isolated Escherichia coli from calves diarrhea | ||||
| International Journal of Advanced Scientific Research and Innovation | ||||
| Volume 5, Issue 2, December 2022, Page 49-71 PDF (424.54 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ijasri.2022.297691 | ||||
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| Authors | ||||
| Ghada .M. Abdel-Hakeem1; Mohamed .W. Abdal-Azeem2; Serageldeen Sultan3; Azhar . M. Hassan4 | ||||
| 1Agriculture Research Center, Animal Health Research Institute, Assuit Branch, Egypt | ||||
| 2Professor- Department of Microbiology, Faculty of Veterinary Medicine, South Valley University | ||||
| 3Professor- Department of Microbiology, Virology division. Faculty of Veterinary Medicine, South Valley University | ||||
| 4Professor- Agriculture Research Center, Animal Health Research Institute, Assuit Branch, Egypt. | ||||
| Abstract | ||||
| Neonatal calf diarrhea (NCD) is a major disease in calves. Escherichia coli is considered the most common and economically important cause of diarrhea in young calves.This work is conducted to determine the extended spectrum ßlactamase (ESBL) producing Escherichia coli (E. coli) isolated from calf diarrhea and select the most effective antibiotic used to treat this problem. A total of two hundred fecal samples of diarrheic calves were collected for the presence of ßlactamase encoding genes (blaTEM, blaSHV, and blaOXA) 84(42%) out of those samples were E.coli positive.The commonly identified E.coli serogroups were O26,O111,O128,O91,O114,O103,O17, and O127. Escherichia coli isolates showed an obstacle of antimicrobial resistance for different antimicrobials while most of these isolates cleared susceptibility for ciprofloxacin and Ceftriaxone. Depending on the results of PCR, the Stx1and Stx2 were negative in all the isolates. Only one isolate O26 carried Stx1 gene and O17 carried Stx2 gene. It was found that all representative serotypes were negative for eae gene except O111, O26, and O127.blaTEM, blaSHV were the most predominant genes (100%) but blaOXA had the lowest prevalence (12.5%). The results proved that multiple antibiotic resistances are widely spread among isolated strains of E. coli. Regular screening of antibiotics sensitivity before actual application to animals is essential to reduce the possibility of dissemination of resistance genes. | ||||
| Keywords | ||||
| Escherichia coli; virulence genes; ꞵ-lactamase; quinolones; calves | ||||
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