A Comparative Histological Study On The Therapeutic Effect of Mesenchymal Stem Cells Versus Exosomes on CCL4 Induced Liver Injury in Adult Male Albino Rats | ||||
| Egyptian Journal of Histology | ||||
| Articles in Press, Accepted Manuscript, Available Online from 07 May 2023 | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejh.2023.206683.1884 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Aya Helal Mahmoud  1; samiha aly mortada2; Mary Attia Morcos3; Asmaa Mohamed Abdel Hameed4
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| 1Histology faculty of medicine cairo university | ||||
| 2faculty | ||||
| 3histology department faculty of medicine cairo university | ||||
| 4Histology departement, faculty of medicine, cairo university, egypt | ||||
| Abstract | ||||
| Background: Liver diseases are major global health burden which lead to many complications if left untreated. Aim: Evaluating and comparing the therapeutic effect of bone marrow mesenchymal stem cells (BMMSCs) versus Exosomes in CCL4 induced liver injury rat model. Methods: Thirty-two adult male albino rats were divided into:Group І (Control),Group II (CCL4), Group III (Recovery),Group IV (MSCs),Group V(Exosomes). 0.1ml/kg of CCL4 in 0.1 ml olive oil was administered intraperitoneally (IP) twice weekly for six weeks for all experimental groups. MSCs were injected in the tail vein of group IV at a dose1×106 after last CCL4 injection. Exosomes were injected in the tail vein of group V at a dose 250 µg after last CCL4 injection. Animals of group II were sacrificed 6 weeks from the start of experiment. while groups(I&III&IV&V) were sacrificed 10 weeks after. ALT&AST were measured at the end of 6thand 10th week. Hematoxylin and eosin, Masson's trichrome stain,α-SMA and Caspase 3 immunostaining were performed. Mean values of AST&ALT,mean area % of collagen, mean number of α-SMA&caspase3 immunopositive cells &mean optical density of caspase 3 immunoreactivity were measured. Results: In groups II& III, hepatocytes showed cytoplasmic vacuolations with dark pyknotic nuclei.A significant increase in mean values of liver enzymes,mean area % of collagen, mean number of α-SMA & caspase 3 immunopositive cells and mean optical density of caspase 3 in group II&III compared to control group and other groups. In group IV&V hepatocytes were apparently normal, few cells showed dark pyknotic nuclei. A significant decrease in mean values of liver enzymes, mean area % of collagen, mean number ofα-SMA &caspase 3 +ve cells and mean optical density of caspase 3 in group IV&V compared to groups II&III.Conclusion: MSCs was effective as Exosomes with significant reduction of fibrosis in liver fibrosis rat model. | ||||
| Keywords | ||||
| MSCs; Exosomes; liver; fibrosis; CCL4 | ||||
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