Anti-Blastocystis in vitro activity and in vivo efficacy of the ethanol extracts of Origanum syriacum L. and Ceratonia siliqua
ALKhalaf, E., Alabdurahman, G., Assany, Y., El-Badry, A. (2023). Anti-Blastocystis in vitro activity and in vivo efficacy of the ethanol extracts of Origanum syriacum L. and Ceratonia siliqua. EKB Journal Management System, 16(1), 73-78. doi: 10.21608/puj.2023.202502.1207
Eiyad ALKhalaf; Ghassan Alabdurahman; Yahia Assany; Ayman El-Badry. "Anti-Blastocystis in vitro activity and in vivo efficacy of the ethanol extracts of Origanum syriacum L. and Ceratonia siliqua". EKB Journal Management System, 16, 1, 2023, 73-78. doi: 10.21608/puj.2023.202502.1207
ALKhalaf, E., Alabdurahman, G., Assany, Y., El-Badry, A. (2023). 'Anti-Blastocystis in vitro activity and in vivo efficacy of the ethanol extracts of Origanum syriacum L. and Ceratonia siliqua', EKB Journal Management System, 16(1), pp. 73-78. doi: 10.21608/puj.2023.202502.1207
ALKhalaf, E., Alabdurahman, G., Assany, Y., El-Badry, A. Anti-Blastocystis in vitro activity and in vivo efficacy of the ethanol extracts of Origanum syriacum L. and Ceratonia siliqua. EKB Journal Management System, 2023; 16(1): 73-78. doi: 10.21608/puj.2023.202502.1207

Anti-Blastocystis in vitro activity and in vivo efficacy of the ethanol extracts of Origanum syriacum L. and Ceratonia siliqua

Parasitologists United Journal
Article 8, Volume 16, Issue 1, April 2023, Page 73-78  XML PDF (408.75 K)
Document Type: Original Article
DOI: 10.21608/puj.2023.202502.1207
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Authors
Eiyad ALKhalaforcid 1; Ghassan Alabdurahman1; Yahia Assany1; Ayman El-Badry* 2
1Departments of Zoology, Faculty of Science, Aleppo University, Syria
2Departments of Microbiology,College of Medicine, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia
Abstract
Background: Blastocystosis is significantly implicated in irritable bowel disease, and its current available
drugs do not provide a complete cure and have several side effects.
Objective: In order to search for a safe natural alternative treatment for blastocystosis, the study
investigated both in vitro activity and in vivo effect of two natural medicinal plants extracts, Origanum
syriacum L. (Marjoram) and Ceratonia siliqua (Carob) on Blastocystis spp. isolated from symptomatic
patients.
Material and Methods: The ethanolic extracts of the two plants, O. syriacum L. and C. siliqua were prepared
in 3 concentrations (0.5, 2.5, 5.0 mg/ml), and screened phytochemically. Blastocystis-positive stool samples
from symptomatic patients were cultured in Jones medium. Cultured Blastocystis cysts were used to screen
the in vitro anti-Blastocystis activity of both plant extracts. Lab-bred mice were experimentally infected
with cultured Blastocystis and treated with different concentrations of the extract of both plants to assess
their in vivo effect. Metronidazole (MTZ) was used as a control drug in both in vitro and in vivo studies.
Results: Ethanolic extract of both plants showed dose and time dependent anti-Blastocystis effects both in
vitro and in vivo. C. siliqua at 5.0 mg/ml showed the highest anti-Blastocystis efficacy and had a maximum
significant growth inhibition rate compared to MTZ.
Conclusion: The ethanolic extract of C. siliqua is a promising, effective, safe, natural, environmentallyfriendly,
anti-Blastocystis alternative therapy. O. syriacum L. had a fair anti-Blastocystis effect and could be
used as a supportive additive.
Keywords
Blastocystis; Ceratonia siliqua (Carob); Origanum syriacum L. (Marjoram); plant extracts; Syria
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