Humoral and Interferon-γ Immune Response to DNA Vaccine Encoding The surface Glycoprotein B of Infectious Laryngotracheitis Virus
gamal, M., Soliman, Y. (2023). Humoral and Interferon-γ Immune Response to DNA Vaccine Encoding The surface Glycoprotein B of Infectious Laryngotracheitis Virus. EKB Journal Management System, 54(4), 631-642. doi: 10.21608/ejvs.2023.204396.1483
Maha Aboelnaga gamal; Yousef Soliman. "Humoral and Interferon-γ Immune Response to DNA Vaccine Encoding The surface Glycoprotein B of Infectious Laryngotracheitis Virus". EKB Journal Management System, 54, 4, 2023, 631-642. doi: 10.21608/ejvs.2023.204396.1483
gamal, M., Soliman, Y. (2023). 'Humoral and Interferon-γ Immune Response to DNA Vaccine Encoding The surface Glycoprotein B of Infectious Laryngotracheitis Virus', EKB Journal Management System, 54(4), pp. 631-642. doi: 10.21608/ejvs.2023.204396.1483
gamal, M., Soliman, Y. Humoral and Interferon-γ Immune Response to DNA Vaccine Encoding The surface Glycoprotein B of Infectious Laryngotracheitis Virus. EKB Journal Management System, 2023; 54(4): 631-642. doi: 10.21608/ejvs.2023.204396.1483

Humoral and Interferon-γ Immune Response to DNA Vaccine Encoding The surface Glycoprotein B of Infectious Laryngotracheitis Virus

Egyptian Journal of Veterinary Sciences
Volume 54, Issue 4 - Serial Number 5, July and August 2023, Page 631-642  XML PDF (1.38 MB)
Document Type: Original Article
DOI: 10.21608/ejvs.2023.204396.1483
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Authors
Maha Aboelnaga gamal email 1; Yousef Solimanorcid 2
1biotechnology department,1Central Laboratory for Evaluation of Veterinary Biologics (CLEVB), Agricultural Research Center {ARC}, Cairo, Egypt
2Central Laboratory for Evaluation of Veterinary Biologics (CLEVB), Agricultural Research Center {ARC}, Cairo.)
Abstract
DNA vaccines continue to be a suitable safe and potent alternative particularly for controlling the infection with pathogens that rely on the cell-mediated type of immune response and for the ability to eliminate viral shedding. Infectious laryngotracheitis virus causes a contagious respiratory disease with a considerable economic impact. DNA vaccine was developed coding for the surface glycoprotein B gene (gpB) from locally isolated strain. The developed vaccine (pcDEST40-gpB) could elicit potent antibody titers positively correlated with the commercially available live TC-propagated ILT vaccine. Cell-mediated immune response (as measured by quantitation of the IFN-γ gene transcript ), revealed that both DNA vaccine and live vaccine initiate a powerful fold change in IFN-γ till the 15 days post-vaccination, however, the use of booster dose of DNA vaccine resulted in an abrupt increase in the level of IFN-γ transcript which was significantly higher than that of the live vaccine by day 7 post-challenge and onward. DNA vaccine but not the live vaccine could eliminate the shedding of the virus post-challenge.
Keywords
Infectious laryngotracheitis- virus (ILTV); DNA vaccine; glycoprotein B gene (gpB)
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