The role of melatonin in potentiation the curative effect of Mesenchymal Stem cells in cuprizone mice Model of Multiple Sclerosis in subcortical white matter tissues. | ||||
| Egyptian Journal of Histology | ||||
| Articles in Press, Accepted Manuscript, Available Online from 11 May 2023 | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejh.2023.193863.1859 | ||||
 View on SCiNiTO | ||||
| Author | ||||
sara mohamed saber  
| ||||
| Department of Histopathology, Egyptian Drug Authority (EDA) | ||||
| Abstract | ||||
| Aim: : The purpose of the study is examination the effects of Bone Marrow Mesenchymal stem cells (BM-MSCs) and melatonin on remyelination in a mouse as a paradigm of multiple sclerosis (MS), both singly and in combination. It is also; investigate if melatonin might enhance BM-MSC activity in subcortical white matter tissues. Methods: 30 mice were categorized into 5 groups. Group (1), control group. The animals of groups (2, 3, 4 and 5) were nourished with food comprising 0.3% cuprizone for 4 weeks for induction the demyelination in subcortical white matter. Group (2) was directly euthanized after 4 weeks of cuprizone feeding. Group (3) the animals were intravenously injected via tail 1x10⁶ BM –MSCs and euthanized after 4 weeks. Group (4) the animals were given intraperitoneal daily doses (5 mg/kg) of melatonin and euthanized after 4 weeks. Group (5) the animals were given BM-MSCs, as in Group (3), and melatonin, as in Group (4), and then euthanized after 4 weeks. Results: The histo-pathological examination of subcortical white matter tissues revealed that cuprizone induced sever alterations in fascicles with notable increase in glial fibrillary acidic protein (GFAP) immunoexpression (IE), loss in sliver impregnation satin, and ultrathin examination showed various morphological changes in myelin sheath. After treatment of animals with BM-MSCs definite improvement could be detected in white matter tissues. Moreover, melatonin administration ameliorates the negative effect of cuprizone. All the findings nearly regressed after coadministration of BM-MSCs + melatonin. Conclusion: BM-MSc and/or melatonin have a potential remylination effect in MS mouse model. Key words: BM-MSc, cuprizone, Melatonin, Multiple sclerosis. | ||||
| Keywords | ||||
| Key words: BM-MSc; cuprizone; Melatonin; Multiple sclerosis | ||||
|
Statistics Article View: 81 |
||||
