Detection of carbapenemase genes and other resistance mechanisms in carbapenem-resistant/cephalosporin-susceptible Pseudomonas aeruginosa
ELSAWY, A., Al-Quthami, K., Abbas, A., Khan, H., Hanawi, W., Hanawi, N., Almowaled, S., Ghazawi, R., Kaki, S., Andejani, S., Felemban, R., Brashi, R., Oqap, E., Sorour, A. (2023). Detection of carbapenemase genes and other resistance mechanisms in carbapenem-resistant/cephalosporin-susceptible Pseudomonas aeruginosa. EKB Journal Management System, 32(3), 13-18. doi: 10.21608/ejmm.2023.298797
ABDELRAHMAN ELSAWY; Khalid Al-Quthami; Ammar Abbas; Hind Khan; Wajanat Hanawi; Naelah Hanawi; Shada Almowaled; Renan Ghazawi; Shirin Kaki; Salwa Andejani; Rakan Felemban; Razaz Brashi; Eyad Oqap; Ashraf Elsayed Sorour. "Detection of carbapenemase genes and other resistance mechanisms in carbapenem-resistant/cephalosporin-susceptible Pseudomonas aeruginosa". EKB Journal Management System, 32, 3, 2023, 13-18. doi: 10.21608/ejmm.2023.298797
ELSAWY, A., Al-Quthami, K., Abbas, A., Khan, H., Hanawi, W., Hanawi, N., Almowaled, S., Ghazawi, R., Kaki, S., Andejani, S., Felemban, R., Brashi, R., Oqap, E., Sorour, A. (2023). 'Detection of carbapenemase genes and other resistance mechanisms in carbapenem-resistant/cephalosporin-susceptible Pseudomonas aeruginosa', EKB Journal Management System, 32(3), pp. 13-18. doi: 10.21608/ejmm.2023.298797
ELSAWY, A., Al-Quthami, K., Abbas, A., Khan, H., Hanawi, W., Hanawi, N., Almowaled, S., Ghazawi, R., Kaki, S., Andejani, S., Felemban, R., Brashi, R., Oqap, E., Sorour, A. Detection of carbapenemase genes and other resistance mechanisms in carbapenem-resistant/cephalosporin-susceptible Pseudomonas aeruginosa. EKB Journal Management System, 2023; 32(3): 13-18. doi: 10.21608/ejmm.2023.298797

Detection of carbapenemase genes and other resistance mechanisms in carbapenem-resistant/cephalosporin-susceptible Pseudomonas aeruginosa

Egyptian Journal of Medical Microbiology
Volume 32, Issue 3, July 2023, Page 13-18  XML PDF (205.9 K)
Document Type: New and original researches in the field of Microbiology.
DOI: 10.21608/ejmm.2023.298797
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Authors
ABDELRAHMAN ELSAWY email 1; Khalid Al-Quthami2; Ammar Abbas2; Hind Khan2; Wajanat Hanawi2; Naelah Hanawi2; Shada Almowaled3; Renan Ghazawi2; Shirin Kaki2; Salwa Andejani3; Rakan Felemban2; Razaz Brashi2; Eyad Oqap2; Ashraf Elsayed Sorourorcid 4
1Medical Microbiology Department, AlAzhar Faculty of medicine, Cairo, Egypt
2Medical Microbiology Laboratory Department, Alnoor Specialist Hospital, Holy Makkah, Saudi Arabia
3Medical Microbiology Laboratory Department, Alnoor Specialist Hospital, Holy Makkah, Saudi Arabi
4Medical Microbiology and Immunology Department, Faculty of medicine – Cairo University, Cairo, Egypt
Abstract
Background: Carbapenem- resistant P. aeruginosa isolates are increasingly observed. Carbapenem resistance in P. aeruginosa is modulated by acquired carbapenemases in association with non-carbapenemases mechanisms. An uncommon phenotype of carbapenem resistant but cephalosporine susceptible (Carb- R/ Ceph- S) Ps. aeruginosa clinical isolates have been reported. Objective: We aimed to assess mechanisms of carbapenem resistant in this characteristic uncommon phenotype. Methodology: A total of 168 carbapenem resistant clinical isolates of Ps. aeruginosa were recovered form admitted cases in a Tertiary Care Hospital during the period from November 2021 to October 2022. All Carb- R/ Ceph- S Pseudomonas aeruginosa isolates were subjected to the following to detect carbapenems resistant mechanism (s):  Genotypic discovery of carbapemenases production, phenotypic detection both of AmpC overproduction and efflux pumps overproduction. Results: 48 isolates (28.6%) were cephalosporine susceptible (Carb-R/ Ceph-S). Genotypic discovery of carbapenemases encoding genes by multiplex PCR and phenotypic detection of AmpC overproduction and efflux pumps overproduction were done to identify the possible mechanisms of carbapenem resistant in the studied phenotypes. None of 48 Carb- R/ Ceph-S P. aeruginosa isolates were carrying carbapenemases encoding genes, 60.4% (29/48) had efflux pumps overproduction and 4.2% (2/48) had AmpC overproduction. The highest rate of antimicrobial resistance was to Tigecycline and Colistin and the smallest rate of antimicrobial resistance was to Piperacillin/ Tazobactam, and Tobramycin and Amikacin. Conclusions: None of Carb-R/ Ceph-S P. aeruginosa harboring carbapenemases encoding genes, whereas;   efflux pumps overproduction and AmpC overproduction were detected in 60.4% and 4.2% respectively.
Keywords
Pseudomonas aeruginosa; carbapenem resistance; Cephalosporine susceptible. carbapenemase; carbapenem resistant/cephalosporine susceptible Ps. aeruginosa
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