Preparation and in-vitro applications of doxorubicin loaded on functionalized multi-walled carbon nanotubes
Toson, E., rezk, N., Zahran, R. (2023). Preparation and in-vitro applications of doxorubicin loaded on functionalized multi-walled carbon nanotubes. EKB Journal Management System, 13(1), 1-12. doi: 10.21608/sjdfs.2023.201890.1096
Elshahat Abo-mosalam Toson; norhan adel rezk; Rasha Fekry Zahran. "Preparation and in-vitro applications of doxorubicin loaded on functionalized multi-walled carbon nanotubes". EKB Journal Management System, 13, 1, 2023, 1-12. doi: 10.21608/sjdfs.2023.201890.1096
Toson, E., rezk, N., Zahran, R. (2023). 'Preparation and in-vitro applications of doxorubicin loaded on functionalized multi-walled carbon nanotubes', EKB Journal Management System, 13(1), pp. 1-12. doi: 10.21608/sjdfs.2023.201890.1096
Toson, E., rezk, N., Zahran, R. Preparation and in-vitro applications of doxorubicin loaded on functionalized multi-walled carbon nanotubes. EKB Journal Management System, 2023; 13(1): 1-12. doi: 10.21608/sjdfs.2023.201890.1096

Preparation and in-vitro applications of doxorubicin loaded on functionalized multi-walled carbon nanotubes

Scientific Journal for Damietta Faculty of Science
Volume 13, Issue 1, June 2023, Page 1-12  XML PDF (701.24 K)
Document Type: Original articles
DOI: 10.21608/sjdfs.2023.201890.1096
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Authors
Elshahat Abo-mosalam Toson email 1; norhan adel rezk2; Rasha Fekry Zahranorcid 3
1Chemistry Department, Faculty of Science, Damietta University, Damietta, New Damietta
2chemistry department, faculty of science, Damietta university, new Damietta
3Chemistry Department (Biochemistry Division), Faculty of Science, Damietta University, Egypt.
Abstract
One of the most crucial nanomaterials used in applications of biomedicine is Multi-Walled Carbon nanotubes (MWCNTs). It is applicable in delivering of different drugs to the tumor target sites, including doxorubicin (DOX). This is because the functionalized MWCNTs can penetrate membranes with low cytotoxicity. In this study, MWCNTs were oxidized (Ox-MWCNTs) to improve their solubility and biocompatibility. Their abilities to encapsulate DOX were tested (Ox-MWCNT/DOX). These particles were then characterized using FTIR, TEM, SEM and zeta size. Their antitumor activities against in-vitroMCF-7 and NBL cells were estimated. The results of FTIR confirmed the addition of carboxyl groups to the MWCNTs followed by encapsulation of DOX.Their shape was tubular with open ends using TEM. From their surface view, SEM showed the tubes as hollow spheres with interconnected structure. Higher releasing rate of DOX at pH 5.8 comparing with pH 7.4 was observed. Treating of human breast cancer cells(MCF-7) with Ox-MWCNT/DOX resulted in cellular viabilities of 96±0.37, 87±0.7, 34.7±0.75, 14.3±1 and 4.2±0.5 % at doses equivalent to 0.1, 1, 10, 100 and 1000µg/ml free DOX, respectively. Moreover, higher viabilities (99.8±0.13, 99.54±0.69, 77.42±1.13, 62.48±0.1 and 55.3±1.25%) with lower cytotoxicities of normal liver cells(NBL) were observed under the same treatment protocol. Therefore, the investigated loading protocol may be a promising one for the treatment of such tumor type after further studies in the future.
Keywords
Carbon nanotubes; Doxorubicin; Drug delivery system; Cytotoxicity
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