Profiling the volatile composition and cytotoxic assessment of the encapsulated Spirulina Platensis using alginate chitosan nanoparticles
Fadl Allah, W., Shemis, M., Abd El Azeem, E., Osman, E., Mamdouh, S. (2023). Profiling the volatile composition and cytotoxic assessment of the encapsulated Spirulina Platensis using alginate chitosan nanoparticles. EKB Journal Management System, 66(12), 511-527. doi: 10.21608/ejchem.2023.199387.7718
Warda M. Fadl Allah; Mohamed Abbas Shemis; Eman M. Abd El Azeem; Ezzat E. A. Osman; Samah Mamdouh. "Profiling the volatile composition and cytotoxic assessment of the encapsulated Spirulina Platensis using alginate chitosan nanoparticles". EKB Journal Management System, 66, 12, 2023, 511-527. doi: 10.21608/ejchem.2023.199387.7718
Fadl Allah, W., Shemis, M., Abd El Azeem, E., Osman, E., Mamdouh, S. (2023). 'Profiling the volatile composition and cytotoxic assessment of the encapsulated Spirulina Platensis using alginate chitosan nanoparticles', EKB Journal Management System, 66(12), pp. 511-527. doi: 10.21608/ejchem.2023.199387.7718
Fadl Allah, W., Shemis, M., Abd El Azeem, E., Osman, E., Mamdouh, S. Profiling the volatile composition and cytotoxic assessment of the encapsulated Spirulina Platensis using alginate chitosan nanoparticles. EKB Journal Management System, 2023; 66(12): 511-527. doi: 10.21608/ejchem.2023.199387.7718

Profiling the volatile composition and cytotoxic assessment of the encapsulated Spirulina Platensis using alginate chitosan nanoparticles

Egyptian Journal of Chemistry
Volume 66, Issue 12, December 2023, Page 511-527  XML PDF (980.68 K)
Document Type: Original Article
DOI: 10.21608/ejchem.2023.199387.7718
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Authors
Warda M. Fadl Allah email 1; Mohamed Abbas Shemis2; Eman M. Abd El Azeemorcid 3; Ezzat E. A. Osman4; Samah Mamdouh2
1Department of Biochemistry and Molecular Biology, Theodor Bilharz Research Institute, Kornaish El-Nile St., 12411, Giza, Egypt
2Department of Biochemistry and Molecular Biology, Theodor Bilharz Research Institute, Kornaish El-Nile St., 12411, Giza, Egypt.
3Department of Biochemistry, Faculty of Science, Ain Shams University, Cairo, Egypt.
4Department of Medicinal Chemistry, Theodor Bilharz Research Institute, Kornaish El-Nile St., 12411, Giza, Egypt.
Abstract
Spirulina platensis (S. platensis) is a blue-green microalga that belongs to the cyanobacteria family. The goal of this research was to investigate the anticancer activity of encapsulation Spirulina platensis algae with Chitosan-Alginate polyelectrolyte nanoparticles on HepG-2, Caco-2, and Vero cell lines and profile its chemical components . S.platensis was extracted using 90% MeOH as a crude extract which was fractionated using different organic solvents. While Nanoencapsulation of 90% MeOH ext. and pet-ether fr. by Chitosan-Alginate polyelectrolyte nanoparticles were prepared by modified ionic gelation method. The chemical composition of 90% MeOH ext. was identified using the GC-MS technique. The 90% MeOH ext. of S. platensis showed high toxicity on HepG-2 and Caco-2 cells, more than the pet-ether fraction. While they were safe on Vero normal cells. Furthermore, nanoencapsulation of the 90%MeOH ext. and pet-ether fr. increased the cytotoxicity against HepG-2 and Caco-2 cells. Moreover, The effect of 90%MeOH ext. and pet-ether fr. NPs against HepG-2 and Caco-2 resulted in a significant increase in the relative expression of the Caspase-3 enzyme and a decrease in the expression of the TNF-α gene and COX-2 enzyme more than the non-encapsulated form. The GC-MS analysis of the silylated 90 % MeOH ext. revealed the presence of 57 bioactive compounds including, alcohols, terpenes, organic acids, and others.
Keywords
anti-cancer; Spirulina platensis; Alginate; nanoencapsulation; GC-MS analysis
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