Molecular studies of virulence genes of Salmonella Typhimurium causing Clinical mastitis in dairy cattle | ||||
| Benha Veterinary Medical Journal | ||||
| Article 4, Volume 33, Issue 2, December 2017, Page 27-37 PDF (994.36 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/bvmj.2017.29985 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Ashraf A. Abd El- Tawab1; Ashraf M. Nabih2; Mohsen A. Agag3; Marwah H. Abd Ali.4 | ||||
| 1Bacteriology, Immunology and Mycology Department, Faculty of Veterinary Medicine, Benha University | ||||
| 2Animal Reproduction Research Institute (ARRI), Haram / Giza | ||||
| 3Theriogenology Department, Faculty of Veterinary Medicine, Benha University | ||||
| 4Veterinary Collage- Banha University | ||||
| Abstract | ||||
| A total of 213 milk samples from clinically mastitic cattle cows were collected from different Governorates of Egypt and transferred in ice box as soon as possible to Bacteriological lab. in Animal Reproduction Research Institute (ARRI) in Giza Governorate (AL-Haram) for bacteriological examination of most important pathogens causing clinical mastitis with special references for isolation and strict identification of Salmonella species. All samples were collected during the period from December 2016 till July 2017 from governorates of Egypt. The bacteriological investigations revealed that 8 (3.7%) of Salmonella isolates were identified biochemically from all examined samples. Serological study showed that a total of 5 (2.3%) of Salmonella isolates were typed as Salmonella Typhimurium. Two strains of Salmonella Typhimurium were isolated singly in the rate of (0.93%) from all examined samples, also another two strains of Salmonella Typhimurium were isolated mixed with Staph aureus in the rate of (0.93%), meanwhile only one strain of same species was isolated mixed with E.coli in the rate of (0.47%). Cefiquinom and Enrofloxacin were sensitively in the rate of (100%), Ampicillin, Chloraphincol, Cloxacillin were resistance in the rate of (100 %) to Streptomycin and Amoxicillin. The molecular examination confirmed that all 5 examined serotyped strains were Salmonella Typhimurium. Virulence genes invA, hilA, avrA, were detected in examined sample by 100%, meanwhile sopE, ssaQ, and fimH genes were not detected by zero%. The objectives of the present study was to investigate the occurrence of Salmonella serotypes as well as to determine the frequency distribution of Six virulence genes (invA, hilA, avrA, ssaQ, sopE and fimH) in salmonella isolates from cattle clinical mastitic milk, in addition to determine the drugs of choice for treatment of most Salmonella strains causing cattle clinical mastitis. | ||||
| Keywords | ||||
| Cattle diseases- Clinical mastitis–Salmonella Infection-Molecular study- Virulence genes | ||||
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